Synthesis and analytical characterisation of copper-based nanocoatings for bioactive stone artworks treatment. Anal Bioanal Chem

Dipartimento di Chimica, Università degli Studi di Bari Aldo Moro, via Orabona 4, 70125 Bari, Italy.
Analytical and Bioanalytical Chemistry (Impact Factor: 3.44). 10/2010; 399(1):473-81. DOI: 10.1007/s00216-010-4301-8
Source: PubMed


Biological agents play an important role in the deterioration of cultural heritage causing aesthetic, biogeophysical and biogeochemical damages. Conservation is based on the use of preventive and remedial methods. The former aims at inhibiting biological attack, and the latter aims at eradicating the biological agents responsible for biodeterioration. Here, we propose the preparation and the analytical characterisation of copper-based nanocoating, capable of acting both as a remedy and to prevent microbial proliferation. Core–shell CuNPs are mixed with a silicon-based product, commonly used as a water-repellent/consolidant, to obtain a combined bioactive system to be applied on stone substrates. The resulting coatings exert a marked biological activity over a long period of time due to the continuous and controlled release of copper ions acting as biocides. To the best of our knowledge, this is the first time that a multifunctional material is proposed, combining the antimicrobial properties of nanostructured coatings with those of the formulations applied to the restoration of stone artworks. A complete characterisation based on a multi-technique analytical approach is presented.

Online abstract figure
Release properties and morphological features of copper-based nanocoatings.

Download full-text


Available from: Inez D van der Werf, Jun 03, 2014
  • Source
    • "Nanoparticles are much more active than larger size particles because of their much higher surface area and ability to display unique physical and chemical properties. The use of nanoparticles as antimicrobial agents for archeological objects has been already proposed and tested, but mainly on stone substrates [7] [8]. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Zinc oxide nanoparticles were prepared and used for surface treatment of oil paintings painted on paper supports. The prepared coating mixture containing 2% of ZnO nanoparticles showed excellent transparency. The effect of coating on protecting the paper support and paintings against microbial attack by Trichoderma reesei and Aspergillus niger, dirt accumulation, and UV aging was studied. Coatings containing ZnO nanoparticles enhanced the durability of linseed oil-based paintings toward UV aging regarding the change in color. Coatings containing ZnO nanoparticles improved resistance to microbial attack when subjected to inoculums containing T. reesei or A. niger fungi. In addition, coatings containing ZnO nanoparticles reduced accumulation of dirt on oil paintings when left in open air for 6 months; cleaning of paintings was quite easy compared to the non-coated paintings or those coated with the varnish without ZnO nanoparticles.
    Journal of Cultural Heritage 03/2014; 15(2):165–172. DOI:10.1016/j.culher.2013.01.012 · 1.57 Impact Factor
    • "Several methods to prevent stone biodegradation were proposed including biocide treatment (Cámara et al., 2011), copper-based nanocoating (Ditaranto et al., 2011). Another approach was proposed to eliminate gram-negative bacteria in the treatment of monument stone by their membrane permeabilization. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Biodegradation is a long process and microorganism communities responsible for this process are complex, mostly composed mainly of fungi and bacteria. Some of these communities, depending on the substrate and micro climatic conditions, may be associated with cyanobacteria, algae and Archaea . Determination of the diversity of microorganisms that inhabit different art works is of major importance for restoration and conservation. The methods used in studying these communities in recent decades can be summarized to a few techniques (DGGE, cultured and uncultured methods, 16S rDNA clone library analysis etc), but these may underestimate the diversity of communities compared to new techniques available (next generation sequencings methods) that provide more close to reality results. In this paper we tried to show the research findings in last few decades that point microorganism communities that populate the world heritage objects and we support the need to investigate these communities using new next generation sequencing methods. Even if conservation can be extended at millennia, preserving for centuries the works of art is a win for world culture.
    Annals of the Romanian Society for Cell Biology 12/2012; 17(2):37-42.
  • [Show abstract] [Hide abstract]
    ABSTRACT: Bioactive copper nanomaterials are an emerging class of nano-antimicrobials providing complimentary effects and characteristics, as compared to other nano-sized metals, such as silver or zinc oxide nanoparticles. In this chapter, copper nano-antimicrobials are reviewed and classified firstly as a function of the preparation methods, and secondly as a function of the target microorganism used for testing their antimicrobial activity. The antimicrobial activity of copper-based nanostructures depends on the microbial species and on the experimental set-up. As a consequence, in this chapter details are provided on methods, as well as on experimental details such as contact time, microorganism strain, concentration of the interacting species, etc. Finally, real-life applications of copper-based nanoantimicrobials are briefly discussed.
    Nano-Antimicrobials, 01/2012: pages 85-117; , ISBN: 978-3-642-24427-8
Show more