Article

Dual plane in-line digital holographic microscopy.

Department of Physics, University of Massachusetts, Boston, Massachusetts 02125, USA.
Optics Letters (impact factor: 3.4). 10/2010; 35(20):3426-8. pp.3426-8
Source: PubMed

ABSTRACT We report a dual plane in-line digital holographic microscopy technique that exploits the method of subtraction of average intensity of the entire hologram to suppress the zero-order diffracted wave. Two interferograms are recorded at different planes to eliminate the conjugate image. The experimental results demonstrate successful reconstruction of phase objects as well as of amplitude objects. The two interferograms can be recorded simultaneously, using two CCD or CMOS sensors, in order to increase the acquisition rate. This enhanced acquisition rate, together with the improved reconstruction capability of the proposed method, may find applications in biomedical research for visualization of rapid dynamic processes at the cellular level.

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Keywords

acquisition rate
 
biomedical research
 
cellular level
 
CMOS sensors
 
different planes
 
dual plane in-line digital holographic microscopy technique
 
enhanced acquisition rate
 
entire hologram
 
exploits
 
rapid dynamic processes
 
subtraction
 
visualization