Evaluation of Spectra VRE, a New Chromogenic Agar Medium Designed To Screen for Vancomycin-Resistant Enterococcus faecalis and Enterococcus faecium

Department of Pathology, Medical College of Wisconsin, and Children's Hospital of Wisconsin, 9200 West Wisconsin Ave., Milwaukee, WI 53226, USA.
Journal of clinical microbiology (Impact Factor: 3.99). 10/2010; 48(12):4627-9. DOI: 10.1128/JCM.01676-10
Source: PubMed


Spectra VRE (Remel, Lenexa, KS) is a chromogenic medium designed to recover and differentiate vancomycin-resistant Enterococcus faecium and Enterococcus faecalis (VRE). This medium was compared to bile esculin azide agar (BEAV) and was 98.2% sensitive and 99.3% specific compared to
BEAV, which was 87.6% sensitive and 87.1% specific at 24 h.

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Available from: Jess Peterson, Oct 04, 2015
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    ABSTRACT: A total of 142 stool specimens were evaluated for vancomycin-resistant enterococcus (VRE). Twenty-four-hour sensitivities and specificities, respectively, were 98% and 95% for Spectra VRE chromogenic agar (Remel, Lenexa, KS), 86% and 92% for bile esculin azide with vancomycin (BEAV; Remel), and 96.5% and 92% for Campylobacter agar (CAMPY; Remel). Spectra VRE and CAMPY are significantly more sensitive at 24 h than BEAV.
    Journal of clinical microbiology 08/2011; 49(11):3947-9. DOI:10.1128/JCM.00180-11 · 3.99 Impact Factor
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    ABSTRACT: Campylobacter (Campy; BD Diagnostics, Sparks, MD), Spectra VRE (Remel, Lenexa, KS), and bile-esculin-azide-vancomycin (BEAV; Remel) agars were compared for their ability to detect vancomycin-resistant enterococci (VRE) in 750 stool specimens. The media were compared at 24 h and 48 h of incubation at 35°C and 42°C. When incubated for 24 h at 35°C, Campy was the most sensitive (97.8%) and specific (99.9%) but was comparable to Spectra, which has a sensitivity of 95.6% and a specificity of 99.1%, whereas BEAV was significantly less sensitive (90%) and specific (96.1%). Incubation at 42°C or extended incubation at 35°C for 48 h yielded no advantage over incubation at 35°C for 24 h.
    Journal of clinical microbiology 04/2012; 50(7):2503-5. DOI:10.1128/JCM.00479-12 · 3.99 Impact Factor
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    ABSTRACT: From November 2011 through March 2012, we surveyed 409 babies in our neonatal intensive care unit for rectal colonization with vancomycin-resistant enterococci. Using Spectra™ VRE media (Remel Diagnostics, Lenexa, KS), we identified one neonate colonized with vancomycin-resistant E. faecium. In addition, 55 (13%) of the surveillance cultures yielded false-positive results with vancomycin-susceptible E. faecalis. During the same time period, 580 rectal swabs were collected from adult patients resulting in 20 (3%) false-positive cultures. The difference in false-positive rates between cultures from babies and adults was statistically significant (p<0.001), prompting an investigation of factors that might influence the elevated false-positive rate in the neonates including patient demographics, nutrition and topical ointments applied at the time of testing. Older neonates, median age of 6 wk, were more likely to have false-positive cultures than younger neonates with median age of 3 wk. (p< 0.001). The younger neonates receiving Similac Expert Care products were less likely to have false-positive surveillance cultures than those receiving other formulas (p<0.001). Application of topical products was not associated with false-positive cultures. The false-positive E. faecalis strains were typed by Diversilab rep-PCR (bioMerieux, Marcy-l'Etoile, France) and found to represent 8 different groups of isolates. The utility of the Spectra™ VRE media appeared to be significantly impacted by the age of the patients screened.
    Journal of clinical microbiology 12/2012; 51(3). DOI:10.1128/JCM.02866-12 · 3.99 Impact Factor
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