Article

The roles of thiol oxidoreductases in yeast replicative aging.

Izmir Institute of Technology, Department of Molecular Biology and Genetics, 35430 Urla, Izmir, Turkey.
Mechanisms of ageing and development (Impact Factor: 4.18). 10/2010; 131(11-12):692-9. DOI: 10.1016/j.mad.2010.09.006
Source: PubMed

ABSTRACT Thiol-based redox reactions are involved in the regulation of a variety of biological functions, such as protection against oxidative stress, signal transduction and protein folding. Some proteins involved in redox regulation have been shown to modulate life span in organisms from yeast to mammals. To assess the role of thiol oxidoreductases in aging on a genome-wide scale, we analyzed the replicative life span of yeast cells lacking known and candidate thiol oxidoreductases. The data suggest the role of several pathways in controlling yeast replicative life span, including thioredoxin reduction, protein folding and degradation, peroxide reduction, PIP3 signaling, and ATP synthesis.

0 Bookmarks
 · 
77 Views
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: We investigated the mechanism underlying the natural variation in longevity within natural populations using the model budding yeast, Saccharomyces cerevisiae. We analyzed whole-genome gene expression in four progeny of a natural S. cerevisiae strain that display differential replicative aging. Genes with different expression levels in short- and long-lived strains were classified disproportionately into metabolism, transport, development, transcription or cell cycle, and organelle organization (mitochondrial, chromosomal, and cytoskeletal). With several independent validating experiments, we detected 15 genes with consistent differential expression levels between the long- and the short-lived progeny. Among those 15, SIR2, HSP30, and TIM17 were upregulated in long-lived strains, which is consistent with the known effects of gene silencing, stress response, and mitochondrial function on aging. The link between SIR2 and yeast natural life span variation offers some intriguing ties to the allelic association of the human homolog SIRT1 to visceral obesity and metabolic response to lifestyle intervention.
    FEMS Yeast Research 02/2011; 11(4):345-55. · 2.46 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Thioredoxins, glutaredoxins, and peroxiredoxins have been characterized as electron donors, guards of the intracellular redox state, and 'antioxidants'. Today, these redoxins are increasingly recognized for their specific role in redox signaling. Redoxin research is by no means 'old-fashioned'; on the contrary, the number of publications on the topic continues to increase exponentially. This review summarizes the almost 50 years of redoxin research, focusing primarily on recent data from vertebrates and mammals. The role of Trx family and related proteins in redox signaling is discussed by looking at reaction mechanisms, reversible oxidative post-translational modifications of proteins, and characterized interaction partners of the redoxins. On basis of this analysis, the importance of the redoxins for human health is addressed in the second part of this review, i.e. their potential impact and functions in different cell types, metabolic and signaling pathways, and various pathological conditions.
    Antioxidants and Redox Signaling 11/2013; 19(13):1539-1605. · 7.19 Impact Factor

Full-text (2 Sources)

View
7 Downloads
Available from
Jun 10, 2014