Unique genome-wide transcriptome profiles of chicken macrophages exposed to Salmonella-derived endotoxin.

Departments of Animal Science, Iowa State University, Ames, Iowa 50011, USA.
BMC Genomics (Impact Factor: 4.4). 10/2010; 11:545. DOI: 10.1186/1471-2164-11-545
Source: PubMed

ABSTRACT Macrophages play essential roles in both innate and adaptive immune responses. Bacteria require endotoxin, a complex lipopolysaccharide, for outer membrane permeability and the host interprets endotoxin as a signal to initiate an innate immune response. The focus of this study is kinetic and global transcriptional analysis of the chicken macrophage response to in vitro stimulation with endotoxin from Salmonella typhimurium-798.
The 38535-probeset Affymetrix GeneChip Chicken Genome array was used to profile transcriptional response to endotoxin 1, 2, 4, and 8 hours post stimulation (hps). Using a maximum FDR (False Discovery Rate) of 0.05 to declare genes as differentially expressed (DE), we found 13, 33, 1761 and 61 DE genes between endotoxin-stimulated versus non-stimulated cells at 1, 2, 4 and 8 hps, respectively. QPCR demonstrated that endotoxin exposure significantly affected the mRNA expression of IL1B, IL6, IL8, and TLR15, but not IL10 and IFNG in HD 11 cells. Ingenuity Pathway Analysis showed that 10% of the total DE genes were involved in inflammatory response. Three, 9.7, 96.8, and 11.8% of the total DE inflammatory response genes were significantly differentially expressed with endotoxin stimulation at 1, 2, 4 and 8 hps, respectively. The NFKBIA, IL1B, IL8 and CCL4 genes were consistently induced at all times after endotoxin treatment. NLRC5 (CARD domain containing, NOD-like receptor family, RCJMB04_18i2), an intracellular receptor, was induced in HD11 cells treated with endotoxin.
As above using an in vitro model of chicken response to endotoxin, our data revealed the kinetics of gene networks involved in host response to endotoxin and extend the known complexity of networks in chicken immune response to Gram-negative bacteria such as Salmonella. The induction of NFKBIA, IL1B, IL8, CCL4 genes is a consistent signature of host response to endotoxin over time. We make the first report of induction of a NOD-like receptor family member in response to Salmonella endotoxin in chicken macrophages.

  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Infection of chicken with Salmonella may lead to a carrier-state characterized by the persistence of bacteria in the ceca for a long period of time and result in their excretion in feces This excretion is the source of contamination of their congeners and food During infection, enterocytes are the primary target cells for Salmonella, the producers of soluble factors which launch immune response and cells which are reciprocally responsive to surrounding immune cells This study used microarrays to compare the gene expression profile during carrier-state of enterocytes purified from infected and control chicks which are either resistant or susceptible to Salmonella Enteritidis carrier-state In total, we identified 271 genes significantly differentially expressed with an absolute fold change greater than 15 A global analysis determined interaction networks between differentially regulated genes Using an a priori approach, our analyses focused on differentially expressed genes which were transcriptionally linked to cytokines playing a major role in the fate of the immune response The expression of genes transcriptionally linked to type I interferon and TGF-β was down-regulated in infected chicks from both lines Gene expression linked to the Th1 axis suggests the latter is inhibited in both lines Finally, the expression of genes linked to IL-4, IL-5 and IL-13 indicates that susceptibility to carrier-state could be associated with a Th2 bias Overall, these results highlight that the response to Salmonella during the acute phase and carrier-state is different and that enterocytes play a central role in this response
    Veterinary Immunology and Immunopathology 01/2014; · 1.88 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Avian pathogenic Escherichia coli (APEC) are responsible for heavy economic losses in poultry industry. Here we investigate DNA methylome of spleen and identify functional DNA methylation changes related to host response to APEC among groups of non-challenged chickens (NC), challenged with mild (MD) and severe pathology (SV). DNA methylation was enriched in the gene bodies and repeats. Promoter and CGIs are hypomethylated. Integration analysis revealed 22, 87, and 9 genes exhibiting inversely changed DNA methylation and gene expression in NC vs. MD, NC vs. SV, and MD vs. SV, respectively. IL8, IL2RB, and IL1RAPL1 were included. Gene network analysis suggested that besides inflammatory response, other networks and pathways such as organismal injury and abnormalities, cell signaling and molecular transport, are probably related to host response to APEC infection. Moreover, methylation changes in cell cycle processes might contribute to the lesion phenotype differences between MD and SV.
    Scientific Reports 01/2014; 4:4299. · 5.08 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: This study was designed to evaluate the effect of bacteriophage on macrophage-mediated inflammatory immune responses against intracellular pathogens. The intracellular survival of nonlysogenic, lysogenic Salmonella Typhimu-rium, and Listeria monocytogenes were evaluated in chicken macrophage HD11 cells treated with Salmonella bacteriophage P22 for 24 h at 37℃. The relative expression of inflammatory mediator-related genes (IL-1β, IL-6, IL-8, IL-10, LITAF, and iNOS) was estimated by using a qPCR. The production of inflammatory factors (IL-1β, IL-6, IL-8, IL-10, TNF-α, and NO) was determined by using ELISA kits. The numbers of invading nonlysogenic S. Typhimurium (ST), lysogenic S. Typhimurium (LY), and L. monocytogenes(LM) in HD11 cells were reduced by 0.90, 0.83, and 1.51 log units, respectively, after 1 h of infection at 37℃. The relative expression levels of inflammatory mediator-encoding genes (IL-8, IL-10, and iNOS) were increased in ST-and LM-infected chicken macrophage HD11 cells treated with P22. The level of NO production was increased to 26.8 μM at LM-infected HD11 cells treated with P22, which corresponded to the reduction of intracellular L. monocytogenes in HD11 cells. The results suggest that the bacteriophage P22 has the potential to reduce the numbers of intracellular pathogens, S. Typhimurium and L. monocytogenes. This study would provide valuable insights into the interaction between bacteriophages and macro-phages and help to develop new strategies for enhancing the microbiological safety in poultry.
    06/2013; 51:96-103.

Full-text (3 Sources)

Available from
May 27, 2014