Article

Draft genome sequence of Streptomyces clavuligerus NRRL 3585, a producer of diverse secondary metabolites.

Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of Korea.
Journal of bacteriology (impact factor: 3.94). 10/2010; 192(23):6317-8. DOI:10.1128/JB.00859-10 pp.6317-8
Source: PubMed

ABSTRACT Streptomyces clavuligerus is an important industrial strain that produces a number of antibiotics, including clavulanic acid and cephamycin C. A high-quality draft genome sequence of the S. clavuligerus NRRL 3585 strain was produced by employing a hybrid approach that involved Sanger sequencing, Roche/454 pyrosequencing, optical mapping, and partial finishing. Its genome, comprising four linear replicons, one chromosome, and four plasmids, carries numerous sets of genes involved in the biosynthesis of secondary metabolites, including a variety of antibiotics.

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Keywords

clavulanic acid
 
genes
 
high-quality draft genome sequence
 
hybrid approach
 
involved Sanger sequencing
 
linear replicons
 
optical
 
plasmids
 
Roche/454 pyrosequencing
 
S. clavuligerus NRRL 3585 strain
 

Ju Yeon Song