Purification, characterization and comparison of phycoerythrins from three different marine cyanobacterial cultures

BRD School of Biosciences, Sardar Patel Maidan, Vadtal Road, Satellite Campus, Post Box No. 39, Sardar Patel University, Vallabh Vidyanagar 388 120, Anand, Gujarat, India.
Bioresource Technology (Impact Factor: 4.49). 09/2010; 102(2):1795-802. DOI: 10.1016/j.biortech.2010.09.025
Source: PubMed


The present study is focused on purification, characterization and comparison of phycoerythrins from three different marine cyanobacterial cultures--hormidium sp. A27 DM, Lyngbya sp. A09 DM and Halomicronema sp. A32 DM. 'Phycoerythrin' was successfully purified and characterized. On SDS-PAGE, the PE purified from all three young cultures showed four bands--corresponding to α and β subunits of each of PE-I and PE-II. However, phycoerythrin purified after prolonged growth of Phormidium sp. A27 DM and Halomicronema sp. A32DM showed only one band corresponding to 14 kDa whereas Lyngbya sp. A09 DM continued to produce uncleaved phycoerythrin. The absorption spectra of purified PEs from all the three young and old cultures showed variations however the fluorescence studies of the purified PEs in all cases gave the emission spectra at around 580 nm. The described work is of great importance to understand the role of phycoerythrin in adapting cyanobacteria to stress conditions.

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    • "A32DM, Pseudanabaena tenuis , Spirulina fusiformis , Arthronema africanum , Calothrix sp., Oscillatoria quadripunctulata , Pseudanabaena sp. Minkova et al. ( 2007 ), Santiago- Santos et al. ( 2004 ), Soni et al. ( 2010 ), Minkova et al. ( 2007 ), Mishra et al. ( 2008 ), Su et al. ( 2010 ), Cano-Europa et al. ( 2010 ), Parmar et al. ( 2011 ) and Mishra et al. ( 2011 ) Sonication Phycoerythrin Cyanosarcina sp. SK40, Phormidium sp. "
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    ABSTRACT: Microalgae, one of the largest global primary producers, are a potential source of bioactive compounds. They are unique in producing superfine chemicals that can be used in various industrial sectors like pharmaceuticals, neutraceuticals and cosmeceuticals. The chapter is intended to provide an insight to two of the most important pigments obtained from them, phycobiliproteins and carotenoids having species specificity which can be used as a chemo- taxonomic marker. Their unique structural properties plays properties play a crucial role in their biological functions. The water soluble phycobiliproteins are used as fluorescent tags in flow cytometry and immunochemistry while liposoluble carotenoids are potential alternatives to synthetic dyes in the food industry.
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    • "Moreover, imperative properties of PBPs like hepato-protective [6], anti-oxidants [7] and anti-inflammatory activity [8], make them very potential macromolecules for therapeutic, diagnostic and pharmacological applications. Various methods have been reported for purification of PE [9], PC [10] and APC [11] but mainly focused on purification of single PBP at a time. Therefore, this study was aiming to purify PE, PC and APC concurrently form Lyngbya sp. "
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    ABSTRACT: The present study probes into the purification of phycobiliproteins, and characterization of their in vitro anti-oxidant activity. Moreover the study also demonstrates the use of antioxidant virtue of phycoerythrin in moderating the phenomenon of aging in Caenorhabditis elegans. Phycoerythrin, phycocyanin and allophycocyanin were purified successfully from Lyngbya sp. A09DM by ammonium sulfate fractionation appended with Triton X-100 intercession. The success of protocol was examined by a series of biochemical characterization like SDS-PAGE, native-PAGE, UV-visible spectroscopy and fluorescence spectroscopy ensuring purity, integrity and functionality of purified phycoerythrin, phycocyanin and allophycocyanin. Purified phycobiliproteins were evaluated for antioxidant and metal ion chelating activity by various in vitro antioxidant assay systems. Results showed significant and dose-dependent antioxidant as well as metal chelating potential of all phycobiliproteins in decreasing order of phycoerythrin > phycocyanin > allophycocyanin. Expansion in lifespan and improvement in pharyngeal pumping of Caenorhabditis elegans were noticed upon pre-treatment with phycoerythrin (100 μg ml−1). Moreover, phycoerythrin mediated increase in worm survival under oxidative stress revealed that the life expansion effect of phycoerythrin on nematode is in part by an action of its antioxidant virtue. These results collectively added up evidence in favor of the ‘Free - radical theory of aging’. The present report, for the first time, describes antioxidant potential of phycoerythrin and its use in extending life-span of Caenorhabditis elegans.
    PROCESS BIOCHEMISTRY 07/2014; 49(10). DOI:10.1016/j.procbio.2014.06.022 · 2.52 Impact Factor
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    • "Phycoerythrins serve as major light-harvesting proteins in numerous cyanobacteria . It extends the light-harvesting capability of the phycobilisome from red region to the green region of the visible light spectrum (Ajayan et al., 2012; Parmar et al., 2011). "
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    ABSTRACT: The present study investigated the effects of several physicochemical parameters on the improvement of phycobiliproteins (especially phycocyanin) synthesis in a newly isolated species of Nostoc sp. Standard BG110 medium was modified to enhance the biomass productivity in different photobioreactors. The initial pH of 8, light intensity of 40μmolm(-2)s(-1), temperature of 35°C, diurnal cycle of 16:8h (light:dark regime), 75.48μM Na2CO3and 17.65mM NaNO3 were found most suitable for the phycobiliproteins synthesis. Cyanobacteria exhibited chromatic adaptation, causing overexpression of phycocyanin in red and phycoerythrin in green light. The maximum phycobiliproteins yield of 0.13gg(-1) dry cell weight was obtained in green light. Phycocyanin was further purified using thin layer chromatography (TLC), anion exchange chromatography and SDS-PAGE (denaturing gel) electrophoresis.
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