Permeability alterations after surgical trauma in normal rabbit peritoneum

Department of Surgery, Larissa University Hospital, Larissa, Greece. kouritas @
European Surgical Research (Impact Factor: 2.47). 09/2010; 45(2):113-9. DOI: 10.1159/000318146
Source: PubMed


To investigate whether surgical trauma in a rabbit adhesion formation model and the administration of normal saline (N/S), icodextrin (ID) and/or dimetindene maleate (DM) changes the permeability of the normal rabbit parietal peritoneum.
A total of 45 female rabbits were operated on for adhesion formation and were euthanized 10 days later. In some rabbits, ID or N/S was instilled intraabdominally during operation, whereas in others DM was infused intravenously. In others, ID plus DM or no agent was used. Specimens were obtained postoperatively and were mounted between Ussing chambers. Amiloride was used to investigate Na(+) channels. Transmesothelial resistance (R(TM)) was determined as a permeability indicator. Results: Amiloride increased the R(TM) of both surfaces. Surgical trauma increased R(TM) and partially inhibited the effect of amiloride. ID and N/S increased R(TM) and inhibited the effect of amiloride. Use of DM did not change R(TM) and did not inhibit the effect of amiloride. Use of ID plus DM slightly increased R(TM), but the effect of amiloride was blocked.
Surgical trauma impairs the permeability of the normal rabbit parietal peritoneum. ID or N/S surmounted this effect, but DM did not, suggesting that surgical trauma is a diffuse process. Antiadhesion measures influence peritoneal physiology.

7 Reads
  • [Show abstract] [Hide abstract]
    ABSTRACT: OBJECTIVES The pleural membrane of the lower pleural cavity has a greater ability to recycle fluid than the pleural membrane of the upper pleural cavity. During lobectomy, the visceral pleura is removed with the lobe, whereas the parietal pleura is traumatized during manipulation. This study investigates variations of the drainage according to the type of lobectomy and its relation to effusion-related complications.METHODS Data of upper and lower lobectomy patients were compared with those of wedge resection patients. All patients were suctioned until totally dry before closure, and one chest tube was left in the hemithorax. The amount of fluid drained per day, the duration of drainage, the length of hospital stay and the morbidity were noted. Student's paired t-test s and Mann-Whitney U-tests were used for comparison; P < 0.05 was defined as statistically significant.RESULTSPatients after lower lobectomy had more fluid drained when compared with patients after upper lobectomy or wedge resection on the first (636 ± 90, 268 ± 75 and 225 ± 62 ml, respectively; P = 0.002) and second postoperative day (464 ± 94, 237 ± 90 and 220 ± 62 ml, respectively; P = 0.046). The drainage tube was removed earlier in patients with upper lobectomy procedures than in patients with lower lobectomy procedures (4.6 ± 0.9 vs 8.1 ± 1.4 days; P = 0.014). Effusion-related complications developed in lower lobectomies with a higher output from the second postoperative day.CONCLUSIONSA larger amount of fluid is drained after removal of the lower lobes, possibly because the important fluid-recycling ability of the lower parts of the cavity is malfunctioning. Early drainage tube removal after lower lobectomy may be reconsidered when taking into account the possibility of effusion-related complications.
    Interactive Cardiovascular and Thoracic Surgery 01/2013; 16(4). DOI:10.1093/icvts/ivs529 · 1.16 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: This study aims to investigate if histamine induces electrochemical alterations in the normal and post-traumatic peritoneum. Peritoneal rabbit specimens were obtained before surgery and 10 days post-operatively and were mounted in Ussing chambers. Histamine solutions were added facing the intra-peritoneal and outer-peritoneal surface. Dimetindene maleate-, cetirizine-, and ranitidine-pretreated specimens were used to investigate histamine receptor involvement, whereas amiloride- and ouabain-pretreated specimens were used to investigate ion transportation blockage involvement. Trans-mesothelial resistance (R TM ) was determined. Histamine-increased R TM intra-peritoneally and decreased it outer-peritoneally. A less intense effect was induced in post-traumatic specimens. Dimetindene maleate, cetirizine, amiloride, and ouabain totally inhibited this effect, whereas ranitidine only had a partial effect. Histamine induces electrochemical alterations in the normal and post-operative peritoneum. This effect is mediated by interaction with histamine receptors, hindering the normal process of ion trans-mesothelial transportation.
    Inflammation 11/2013; 37(2). DOI:10.1007/s10753-013-9767-z · 2.21 Impact Factor

Similar Publications