Tin oxide nanoparticles-polymer modified single-use sensors for electrochemical monitoring of label-free DNA hybridization

Ege University, Faculty of Pharmacy, Analytical Chemistry Dept., Bornova, 35100 Izmir, Turkey.
Talanta (Impact Factor: 3.51). 10/2010; 82(5):1680-6. DOI: 10.1016/j.talanta.2010.07.040
Source: PubMed

ABSTRACT In this study, SnO(2) nanoparticles (SNPs)-poly(vinylferrocenium) (PVF(+)) modified single-use graphite electrodes were developed for electrochemical monitoring of DNA hybridization. The surfaces of polymer modified and polymer-SNP modified pencil graphite electrodes (PGEs) were firstly characterized by using SEM analysis. The electrochemical behaviours of these electrodes were also investigated using the differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS) techniques. The polymer-SNP modified PGEs were then tested for the electrochemical sensing of DNA based on the changes at the guanine oxidation signals. Experimental parameters, such as; different modifications in DNA oligonucleotides, DNA probe concentrations were examined to obtain more sensitive and selective electrochemical signals for nucleic acid hybridization. After optimization studies, DNA hybridization was investigated in the case of complementary of hepatitis B virus (HBV) probe, mismatch (MM), and noncomplementary (NC) sequences.

  • [Show abstract] [Hide abstract]
    ABSTRACT: The electrooxidation of ethylene glycol (EG) on the surface of gold nanoparticles (AuNPs) in alkaline medium was investigated. AuNPs were electrodeposited on pencil graphite (PG) by fast scan cyclic voltammetry. Different sizes of AuNPs deposited on the surface of PG (AuNPs/PG) were used for the electrooxidation process. AuNPs were electrodeposited on PG at various deposition times in the same potential range but with different scan rates and scan cycles. Scanning electron microscopy (SEM), transmission electron microscopy (TEM) and X-ray diffraction (XRD) were used to visualize and characterize the prepared AuNPs/PG electrodes. Cyclic voltammograms were also used to investigate the electrooxidation of EG. The effects of EG and supporting electrolyte concentrations, scan rate, particle size of AuNPs and final potential limit on the electrooxidation process have been investigated. Further studies showed that the electrooxidation of EG is affected by temperature of the medium. The prepared AuNPs showed stability after long-term use.
    Science China-Chemistry 02/2011; 55(2). DOI:10.1007/s11426-011-4402-z · 1.52 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Interest in electrochemical analysis of purine nucleobases and few other important purine derivatives has been growing rapidly. Over the period of the past decade, the design of electrochemical biosensors has been focused on achieving high sensitivity and efficiency. The range of existing electrochemical methods with carbon electrode displays the highest rate in the development of biosensors. Moreover, modification of electrode surfaces based on nanomaterials is frequently used due to their extraordinary conductivity and surface to volume ratio. Different strategies for modifying electrode surfaces facilitate electron transport between the electrode surface and biomolecules, including DNA, oligonucleotides and their components. This review aims to summarize recent developments in the electrochemical analysis of purine derivatives, as well as discuss different applications.
    Sensors 01/2015; 15(1):1564-1600. DOI:10.3390/s150101564 · 2.05 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: The effect of the SnO(2) nanoparticles (SNPs) on the behaviour of voltammetric carbon paste electrodes were studied for possible use of this material in biosensor development. The electrochemical behaviour of SNP modified carbon paste electrodes (CPE) was first investigated by using cyclic voltammetry (CV), differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS) techniques. The performance of the SNP modified electrodes were compared to those of unmodified ones and the parameters affecting the response of the modified electrode were optimized. The SNP modified electrodes were then tested for the electrochemical sensing of DNA purine base adenine to explore their further development in biosensor applications.
    Colloids and surfaces B: Biointerfaces 08/2011; 86(1):154-7. DOI:10.1016/j.colsurfb.2011.03.034 · 4.29 Impact Factor