Article

Fluorescent nanoparticles for observing primo vascular system along sciatic nerve.

Biomedical Physics Laboratory, Department of Physics and Astronomy, Seoul National University, 599 Gwanak, Gwanak-gu, Seoul, Korea.
Journal of acupuncture and meridian studies 09/2010; 3(3):150-5. DOI:10.1016/S2005-2901(10)60030-1 pp.150-5
Source: PubMed

ABSTRACT The primo vascular system was found in the epineurium along the rat sciatic nerve following subcutaneous injection of fluorescent nanoparticles at the Zusanli acupoint (ST-36). Nanoparticles were injected into the primo-vessel near ST-36 and flowed along the sciatic nerve. Fluorescence revealed a structure in the epineurium that was hardly detectable. Images of the isolated sample stained with 4',6-diamidino-2-phenylindole were captured using confocal microscopy. These images showed the distinctive nuclei distribution and multi-lumen structure of primo-vessels that differentiate them from lymphatic vessels, blood capillaries and nerves. This study demonstrates a new use for nanoparticles in fluorescence reflectance imaging techniques during in vivo imaging of primo-vessels.

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Keywords

distinctive nuclei distribution
 
epineurium
 
Fluorescence
 
fluorescence reflectance imaging techniques
 
fluorescent nanoparticles
 
Images
 
lymphatic vessels
 
Nanoparticles
 
nerves
 
primo vascular system
 
primo-vessel
 
primo-vessels
 
subcutaneous injection
 
vivo imaging
 
Zusanli acupoint