Connective tissue growth factor expression is increased in collagenous colitis and coeliac disease

Medical Clinic I Gastroenterology, Infectious Diseases, Rheumatology, Charité-Campus Benjamin Franklin, Berlin, Germany.
Histopathology (Impact Factor: 3.45). 09/2010; 57(3):427-35. DOI: 10.1111/j.1365-2559.2010.03652.x
Source: PubMed


Subepithelial collagen deposition is a classical feature of collagenous colitis (CC), but is also seen in untreated coeliac disease. The end-stage mediator of excess cellular collagen production is connective tissue growth factor (CTGF). The aim of this study was to investigate CTGF expression by in situ hybridization (ISH) and polymerase chain reaction (PCR) in CC and coeliac disease as well as lymphocytic colitis (LC), Crohn's colitis and ulcerative colitis (UC).
For coeliac disease we analysed fresh frozen material by quantitative reverse transcription-polymerase chain reaction (RT-PCR) and archival material for ISH. PCR transcripts in coeliac disease were moderately elevated and labelled cells were significantly increased in the subepithelial zone. For CC, LC and UC we investigated archival material because of the rarity of the first two conditions. There was a marked increase in CTGF expression in the subepithelial zone in CC, localizing to cells with the morphology of smooth muscle cells, which was not seen in LC.
The colocalization of CTGF transcripts with areas of excessive collagen deposition in coeliac disease and CC suggest that it might be the end-stage mediator of local fibrosis in these conditions.

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    • "It has been shown that CTFG induces the production of extracellular matrix by stimulating transcription of type 1 collagen [8]. Also recent reports have shown higher CTFG expression in cases with increased connective tissue in lungs, bowel, kidney and liver [9] [10] [11] [12] [13] [14] [15]. Also another recent study has found increased expression of CTFG correlating with severity of fibrosis independent of inflammation in patients with chronic hepatitis C [12]. "
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