Article

Motile and non-motile sperm diagnostic manipulation using optoelectronic tweezers

Department of Electrical Engineering, University of Hawaii at Manoa, Honolulu, HI 96822, USA.
Lab on a Chip (Impact Factor: 5.75). 12/2010; 10(23):3213-7. DOI: 10.1039/c0lc00072h
Source: PubMed

ABSTRACT Optoelectronic tweezers was used to manipulate human spermatozoa to determine whether their response to OET predicts sperm viability among non-motile sperm. We review the electro-physical basis for how live and dead human spermatozoa respond to OET. The maximal velocity that non-motile spermatozoa could be induced to move by attraction or repulsion to a moving OET field was measured. Viable sperm are attracted to OET fields and can be induced to move at an average maximal velocity of 8.8 ± 4.2 µm s(-1), while non-viable sperm are repelled to OET, and are induced to move at an average maximal velocity of -0.8 ± 1.0 µm s(-1). Manipulation of the sperm using OET does not appear to result in increased DNA fragmentation, making this a potential method by which to identify viable non-motile sperm for assisted reproductive technologies.

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