Motile and non-motile sperm diagnostic manipulation using optoelectronic tweezers
ABSTRACT Optoelectronic tweezers was used to manipulate human spermatozoa to determine whether their response to OET predicts sperm viability among non-motile sperm. We review the electro-physical basis for how live and dead human spermatozoa respond to OET. The maximal velocity that non-motile spermatozoa could be induced to move by attraction or repulsion to a moving OET field was measured. Viable sperm are attracted to OET fields and can be induced to move at an average maximal velocity of 8.8 ± 4.2 µm s(-1), while non-viable sperm are repelled to OET, and are induced to move at an average maximal velocity of -0.8 ± 1.0 µm s(-1). Manipulation of the sperm using OET does not appear to result in increased DNA fragmentation, making this a potential method by which to identify viable non-motile sperm for assisted reproductive technologies.
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ABSTRACT: This paper reports on an optoelectrofluidic platform which consists of the organic photoconductive material, titanium oxide phthalocyanine (TiOPc), and the photocrosslinkable polymer, poly (ethylene glycol) diacrylate (PEGDA). TiOPc simplifies the fabrication process of the optoelectronic chip due to requiring only a single spin-coating step. PEGDA is applied to embed the moldless PEGDA-based microchannel between the top ITO glass and the bottom TiOPc substrate. A real-time control interface via a touch panel screen is utilized to select the target 15 μm polystyrene particles. When the microparticles flow to an illuminating light bar, which is oblique to the microfluidic flow path, the lateral driving force diverts the microparticles. Two light patterns, the switching oblique light bar and the optoelectronic ladder phenomenon, are designed to demonstrate the features. This work integrating the new material design, TiOPc and PEGDA, and the ability of mobile microparticle manipulation demonstrates the potential of optoelectronic approach.Advances in OptoElectronics 01/2011; Volume 2011(Article ID 394683). DOI:10.1155/2011/394683
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ABSTRACT: Hybrid opto-electric manipulation in microfluidics/nanofluidics refers to a set of methodologies employing optical modulation of electrokinetic schemes to achieve particle or fluid manipulation at the micro- and nano-scale. Over the last decade, a set of methodologies, which differ in their modulation strategy and/or the length scale of operation, have emerged. These techniques offer new opportunities with their dynamic nature, and their ability for parallel operation has created novel applications and devices. Hybrid opto-electric techniques have been utilized to manipulate objects ranging in diversity from millimetre-sized droplets to nano-particles. This review article discusses the underlying principles, applications and future perspectives of various hybrid opto-electric techniques that have emerged over the last decade under a unified umbrella.Lab on a Chip 07/2011; 11(13):2135-48. DOI:10.1039/c1lc20208a · 5.75 Impact Factor
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ABSTRACT: Magnetic beads are widely utilized for separating biomolecules, DNA and RNA. Traditionally, bulk magnet is utilized for manipulation these particles but when it comes to microscale bulk magnet is not the efficient method. Here, we utilize an organic photoconductive material, TiOPc, to generate light-induced electro-osmosis flow on chip. The fabrication process is convenient to be handled by the researchers and biologists without cleanroom IC fabrication facility. When specifically designed light pattern is projected onto the TiOPc substrate, the conductivity of the organic material layer within the illuminating region increases and the charges are locally assembled on its surface to form a virtual electrode. With an external ac voltage of 5 Vpp at 10 kHz, numerous magnetic beads are attracted from the nonilluminating region toward the center of light-pattern illuminating region. Driven by the moving light image, the grouped magnetic beads can be manipulated and merged in a desired way or direction. The light manipulation process provides a flexible and convenient approach for in vitro control of magnetic beads. We expect that this light-driven technology would display a multifunctional platform for manipulation of microparticles.Magnetics, IEEE Transactions on; 11/2011