Systems biology: The cost of feedback control
(Impact Factor: 41.46).
09/2010; 467(7312):163-4. DOI: 10.1038/467163a
Noise in biochemical processes can compromise precision in cellular functions. An analysis involving information theory suggests that there is a strict limit to how far noise can be suppressed by feedback.
Available from: David L. Abel
The First Gene: The Birth of Programming, Messaging and Formal Control, Edited by D. L. Abel, 01/2011; LongView Press--Academic, Biol. Res. Division, New York, N.Y..
Available from: Shawn M Gomez
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ABSTRACT: Timely, and sometimes rapid, metabolic adaptation to changes in food supply is critical for survival as an organism moves from the fasted to the fed state, and vice versa. These transitions necessitate major metabolic changes to maintain energy homeostasis as the source of blood glucose moves away from ingested carbohydrates, through hepatic glycogen stores, towards gluconeogenesis. The integration of hepatic glycogen regulation with extra-hepatic energetics is a key aspect of these adaptive mechanisms. Here we use computational modeling to explore hepatic glycogen regulation under fed and fasting conditions in the context of a whole-body model. The model was validated against previous experimental results concerning glycogen phosphorylase a (active) and glycogen synthase a dynamics. The model qualitatively reproduced physiological changes that occur during transition from the fed to the fasted state. Analysis of the model reveals a critical role for the inhibition of glycogen synthase phosphatase by glycogen phosphorylase a. This negative regulation leads to high levels of glycogen synthase activity during fasting conditions, which in turn increases substrate (futile) cycling, priming the system for a rapid response once an external source of glucose is restored. This work demonstrates that a mechanistic understanding of the design principles used by metabolic control circuits to maintain homeostasis can benefit from the incorporation of mathematical descriptions of these networks into "whole-body" contextual models that mimic in vivo conditions.
PLoS Computational Biology 12/2011; 7(12):e1002272. DOI:10.1371/journal.pcbi.1002272 · 4.62 Impact Factor
Available from: Chieh Hsu
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ABSTRACT: During evolution, genetic networks are rewired through strengthening or weakening their interactions to develop new regulatory schemes. In the galactose network, the GAL1/GAL3 paralogues and the GAL2 gene enhance their own expression mediated by the Gal4p transcriptional activator. The wiring strength in these feedback loops is set by the number of Gal4p binding sites. Here we show using synthetic circuits that multiplying the binding sites increases the expression of a gene under the direct control of an activator, but this enhancement is not fed back in the circuit. The feedback loops are rather activated by genes that have frequent stochastic bursts and fast RNA decay rates. In this way, rapid adaptation to galactose can be triggered even by weakly expressed genes. Our results indicate that nonlinear stochastic transcriptional responses enable feedback loops to function autonomously, or contrary to what is dictated by the strength of interactions enclosing the circuit.
Nature Communications 02/2012; 3:682. DOI:10.1038/ncomms1687 · 11.47 Impact Factor
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