Pyramidal Neurons Are Generated from Oligodendroglial Progenitor Cells in Adult Piriform Cortex

Institute for Pediatric Regenerative Medicine, University of California, Davis School of Medicine, Sacramento, California 95817, USA.
The Journal of Neuroscience : The Official Journal of the Society for Neuroscience (Impact Factor: 6.34). 09/2010; 30(36):12036-49. DOI: 10.1523/JNEUROSCI.1360-10.2010
Source: PubMed


Previous studies have shown that oligodendroglial progenitor cells (OPCs) can give rise to neurons in vitro and in perinatal cerebral cortex in vivo. We now report that OPCs in adult murine piriform cortex express low levels of doublecortin, a marker for migratory and immature neurons. Additionally, these OPCs express Sox2, a neural stem cell marker, and Pax6, a transcription factor characteristic of progenitors for cortical glutamatergic neurons. Genetic fate-mapping by means of an inducible Cre-LoxP recombination system proved that these OPCs differentiate into pyramidal glutamatergic neurons in piriform cortex. Several lines of evidence indicated that these newly formed neurons became functionally integrated into the cortical neuronal network. Our data suggest that NG2(+)/PDGFRα(+) proteolipid protein promoter-expressing progenitors generate pyramidal glutamatergic neurons within normal adult piriform cortex.

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    • "yama et al . , 2014 ) . The confusion is due in large part to the cell ' s multipotency . While predominantly a pool for OLs – and hence considered OPCs – NG2 - expressing cells also generate protoplasmic astrocytes and a small number of region - specific neurons ( though the latter is hotly debated ) ( Rivers et al . , 2008 ; Zhu et al . , 2008 ; Guo et al . , 2010 ; Kang et al . , 2010 ) . Recent evidence further suggests that NG2 cells are capable of receiving and reacting to neuronal input , though no defini - tive biological function has been ascribed [ reviewed by Hill and Nishiyama ( 2014 ) ] . To generate consensus and acknowledge the NG2 population as distinct , an umbrella term , polydendr"
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    • "These data suggest that a pool of DCX (+) progenitors residing in the piriform cortex may be induced to differentiate in response to injury in accord with previous findings 12. It is possible that several mechanisms are responsible for the reconstitution of layer II neurons: the mobilization of a quiescent population of DCX (+) progenitors residing in the piriform cortex, lesion-induced de-novo neurogenesis in the SVZ followed by migration to the piriform cortex, and, possibly, the maturation of oligodendrocytes progenitor cells into mature neurons 26. On the basis of the data presented here and reports in the literature, we believe that activated DCX (+) progenitors mature into NeuN (+) neurons that reconstitute layer II. "
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    • "The neuronal fate of NG2 cells has been one of the most highly debated topics, and Cre-loxP-mediated genetic fate mapping studies have produced inconsistent findings. For example, two studies using Pdgfra-CreER or PLP (proteolipid protein)-CreER transgenic mouse lines observed reporter+ neurons in the piriform cortex (Rivers et al., 2008; Guo et al., 2010), while a subsequent study using an independent line of Pdgfra-CreER mice did not find any evidence for a neuronal fate (Kang et al., 2010). Earlier studies using NG2- and Olig2-Cre driver mice showed no evidence for neurogenesis (Dimou et al., 2008; Zhu et al., 2008; Komitova et al., 2009; Zhu et al., 2011), while a recent study using the same NG2-creER mice showed a few reporter+ neurons in the hypothalamus (Robins et al., 2013). "
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