Selection and characterization of a multivalent Salmonella phage and its production in a nonpathogenic Escherichia coli strain.

Institute for Biotechnology and Bioengineering, Center of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4700-057 Braga, Portugal.
Applied and Environmental Microbiology (Impact Factor: 3.95). 11/2010; 76(21):7338-42. DOI: 10.1128/AEM.00922-10
Source: PubMed

ABSTRACT We report the selection and amplification of the broad-host-range Salmonella phage phi PVP-SE1 in an alternative nonpathogenic host. The lytic spectrum and the phage DNA restriction profile were not modified upon replication in Escherichia coli Bl21, suggesting the possibility of producing this phage in a nonpathogenic host, contributing to the safety and easier approval of a product based on this Salmonella biocontrol agent.

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May 21, 2014