Diminution of Oxalate Induced Renal Tubular Epithelial Cell Injury and Inhibition of Calcium Oxalate Crystallization in vitro by Aqueous Extract of Tribulus terrestris

Department of Biotechnology and Bioinformatics, Waknaghat, Solan, India.
International braz j urol: official journal of the Brazilian Society of Urology (Impact Factor: 0.88). 08/2010; 36(4):480-8; discussion 488, 489. DOI: 10.1590/S1677-55382010000400011
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Recurrence and persistent side effects of present day treatment for urolithiasis restrict their use, so an alternate solution, using phytotherapy is being sought. The present study attempted to evaluate the antilithiatic properties of Tribulus terrestris commonly called as "gokhru" which is often used in ayurveda to treat various urinary diseases including urolithiasis.
The activity of Tribulus terrestris was investigated on nucleation and the growth of the calcium oxalate (CaOx) crystals as well as on oxalate induced cell injury of NRK 52E renal epithelial cells.
Tribulus terrestris extract exhibited a concentration dependent inhibition of nucleation and the growth of CaOx crystals. When NRK-52E cells were injured by exposure to oxalate for 72 h, Tribulus terrestris extract prevented the injury in a dose-dependent manner. On treatment with the different concentrations of the plant, the cell viability increased and lactate dehydrogenase release decreased in a concentration dependent manner.
The current data suggests that Tribulus terrestris extract not only has a potential to inhibit nucleation and the growth of the CaOx crystals but also has a cytoprotective role. Our results indicate that it could be a potential candidate for phytotherapy against urolithiasis.

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Available from: Simran Tandon, Apr 17, 2015
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    • "The most vite, whewelite, actors in kidney stone formation nly due to the side effects of portant concern allopathic and in India, due to vedic system of nabhedaÊ which es [4]. Various s [6], Herrniaria resteris [9], Flos ntive as well as are studying the as a preventive edicine, which is "
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    • "Media was supplemented with 1% Penicillin (100 units/mL)-Streptomycin (10,000 µg/mL) and 10% fetal bovine serum. Cells were cultured in 25 cm2 tissue-culture treated flasks at 37°C and 5% CO2 in humidified chambers [20]. "
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