Article

Analysis of MAP kinases by hydrogen exchange mass spectrometry.

Department of Chemistry and Biochemistry, University of Colorado, Boulder, CO, USA.
Methods in molecular biology (Clifton, N.J.) 01/2010; 661:239-55. DOI:10.1007/978-1-60761-795-2_14 pp.239-55
Source: PubMed

ABSTRACT Hydrogen exchange mass spectrometry (HX-MS) is an experimental technique that can be used to -examine solvent accessibility and conformational mobility in biological macromolecules. This chapter summarizes studies using HX-MS to examine the regulation of conformation, protein mobility, and ligand binding to MAP kinases. We describe the planning and design of HX-MS experiments, strategies for data analysis and interpretation, and available software.

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Keywords

available software
 
chapter summarizes studies
 
conformation
 
conformational mobility
 
experimental technique
 
HX-MS
 
HX-MS experiments
 
Hydrogen exchange mass spectrometry
 
ligand binding
 
MAP kinases