Evaluating genome-scale approaches to eukaryotic DNA replication.

Department of Biological Science, Florida State University, Tallahassee, 32306, USA.
Nature Reviews Genetics (Impact Factor: 41.06). 10/2010; 11(10):673-84. DOI: 10.1038/nrg2830
Source: PubMed

ABSTRACT Mechanisms regulating where and when eukaryotic DNA replication initiates remain a mystery. Recently, genome-scale methods have been brought to bear on this problem. The identification of replication origins and their associated proteins in yeasts is a well-integrated investigative tool, but corresponding data sets from multicellular organisms are scarce. By contrast, standardized protocols for evaluating replication timing have generated informative data sets for most eukaryotic systems. Here, I summarize the genome-scale methods that are most frequently used to analyse replication in eukaryotes, the kinds of questions each method can address and the technical hurdles that must be overcome to gain a complete understanding of the nature of eukaryotic replication origins.

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