Clinical Microfluidics for Neutrophil Genomics and Proteomics

Department of Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA.
Nature medicine (Impact Factor: 27.36). 09/2010; 16(9):1042-7. DOI: 10.1038/nm.2205
Source: PubMed


Neutrophils have key roles in modulating the immune response. We present a robust methodology for rapidly isolating neutrophils directly from whole blood with 'on-chip' processing for mRNA and protein isolation for genomics and proteomics. We validate this device with an ex vivo stimulation experiment and by comparison with standard bulk isolation methodologies. Last, we implement this tool as part of a near-patient blood processing system within a multi-center clinical study of the immune response to severe trauma and burn injury. The preliminary results from a small cohort of subjects in our study and healthy controls show a unique time-dependent gene expression pattern clearly demonstrating the ability of this tool to discriminate temporal transcriptional events of neutrophils within a clinical setting.

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    • "The potential problem with these studies is that isolation may affect gene expression (Holmes et al, 2009; Kotz et al, 2010; Lee et al, 2010; Shim et al, 2010). This may be solved by microfluiditic devices that use cell-specific antibodies that isolate specific cell types at the bed side (Kotz et al, 2010). "
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