Pluripotent stem cell-derived natural killer cells for cancer therapy

Department of Medicine and Stem Cell Institute, University of Minnesota, Minneapolis, MN 55455, USA.
Translational research : the journal of laboratory and clinical medicine 09/2010; 156(3):147-54. DOI: 10.1016/j.trsl.2010.07.008
Source: PubMed

ABSTRACT Human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) provide an accessible, genetically tractable, and homogenous starting cell population to efficiently study human blood cell development. These cell populations provide platforms to develop new cell-based therapies to treat both malignant and nonmalignant hematological diseases. Our group previously demonstrated the ability of hESC-derived hematopoietic precursors to produce functional natural killer (NK) cells as well as an explanation of the underlying mechanism responsible for the inefficient development of T and B cells from hESCs. hESCs and iPSCs, which can be engineered reliably in vitro, provide an important new model system to study human lymphocyte development and produce enhanced cell-based therapies with the potential to serve as a "universal" source of antitumor lymphocytes. This review will focus on the application of hESC-derived NK cells with currently used and novel therapeutics for clinical trials, barriers to translation, and future applications through genetic engineering approaches.

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Available from: Dan S Kaufman, Nov 17, 2014
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    • "This approach could generate novel insights into NK cell turnover, differentiation, migratory behavior , in vivo killing of target cells, and other areas. Moreover, induced pluripotent stem cells have been used to generate hNK cells with antiviral activity against HIV (Knorr and Kaufman, 2010). NK cells derived from induced rhesus pluripotent stem cells (Liu et al., 2008) could be used to monitor in vivo NK cell development and differentiation. "
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