Antimicrobial Properties of Derris scandens Aqueous Extract

Journal of Biological Sciences 06/2009; 9(6). DOI: 10.3923/jbs.2009.607.611
Source: DOAJ

ABSTRACT The objective of this study is to evaluate the antimicrobial activity of D. scandens aqueous extract against various bacteria. The stems of D. scandens were extracted using aqueous extraction with yield 1.5-2% of dried weight of plant stems. The antimicrobial activity was screened by using agar diffusion method. Minimum Inhibitory Concentrations (MICs) and MBCs values were determined using agar dilution method and broth macro-dilution method. The agar diffusion tested revealed that the plant aqueous extract showed inhibition zone against S. aureus ATCC 25923, S. epidermidis ATCC 12228 and E. coli ATCC 25922. Among three bacteria, S. aureus and S. epidermidis is gram positive bacteria, while E. coli is gram negative bacteria. Minimum Bactericial Concentration (MICs) of the plant aqueous extract were in range of 2-4 g L-1, while MBCs were in the range of 4-16 g L-1. In conclusion, the plant extract showed good inhibitory effect on growth of S. aureus and E. coli which are nosocomial infection bacteria and those S. epidermidis which is normal flora.

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    ABSTRACT: The current study was aimed to investigate pancreatic protective and anti-diabetic activities of the aqueous extract of Derris reticulata stem. First, we evaluated a cytoprotective potential of D. reticulata extract on alloxan-induced damage in vitro. Treatment with D. reticulata extract at the doses of 250 and 500 μg/ml significantly increased cell viability of the pancreatic β-cell line RINm5F after exposure of alloxan. The anti-hyperglycemic activity of D. reticulata extract was further studied in alloxan-induced diabetic rats. A significant reduction in blood glucose level along with an increase in body weight was observed in diabetic rats treated with D. reticulata extract at 250 mg/kg body weight for 15 days. Serum aspartate transaminase and alanine transaminase levels were also significantly decreased compared to diabetic control rats. In accordance with in vitro cytoprotective effect, histopathological examination revealed that pancreatic islet cells of the extract-treated diabetic rat were less damage than those of the untreated diabetic group. In order to find another possible mechanism of action underling hypoglycemic activity, the effect on glucose absorption was examined using everted sac jejunum. The results showed that D. reticulata extract suppressed glucose absorption from small intestine. To corroborate safety use of D. reticulata extract, acute oral toxicity was also conducted in rats. Our results showed that none of the tested doses (250, 500, 1,000, and 2,000 mg/kg) induced signs of toxicity or mortality after administration of the extract. The results suggested that D. reticulata extract possess anti-diabetic activity, which resulting from its pancreatic cytoprotective effect and inhibition of intestinal glucose absorption.
    Journal of physiology and biochemistry 05/2014; 70(3). DOI:10.1007/s13105-014-0337-0 · 2.50 Impact Factor
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    ABSTRACT: Different fractions of root and stem of Derris scandens demonstrated good antibacterial (Escherichia coli, and Bacillus megaterium), antialgal (Chlorella fusca), and antifungal (Microbotryum violaceum) activity. Phytochemical investigation resulted in isolation of scandenin, scandenin A, betulinic acid, lupeol, β-amyran-3-one, β-amyrin, β-sitosterol and ß-sitosterol glucopyranoside. Study showed that scandenin has strong antibacterial activity against B. megaterium and good antifungal and antialgal properties. Scandenin A showed good antibacterial, antifungal and antialgal properties.
    Journal of King Saud University - Science 01/2015; 4. DOI:10.1016/j.jksus.2015.01.001
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    Post-genomic Approaches in Drug and Vaccine Development, First 11/2014: chapter 13: pages 333-367; River Publishers.


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