Article

A nanoparticle dispersion method for in vitro and in vivo nanotoxicity study.

Department of Mechanical Engineering, University of Minnesota, 111 Church Street SE, Minneapolis, MN 55455, USA.
Nanotoxicology (impact factor: 5.76). 03/2010; 4(1):42-51. DOI:10.3109/17435390903374019 pp.42-51
Source: PubMed

ABSTRACT The dispersion in air of nanoparticles of different sizes, materials and morphologies with controlled agglomeration involving aerosol delivery for in vivo and in vitro studies is one of the most difficult challenges in the field of nanoparticle toxicology. We describe here a nanoparticle dispersion system using an electrospray method to deliver airborne nanoparticles (approximately 10-100 nm) with spatial uniformity and controllable particle concentration for in vitro and in vivo studies. With the dispersion method, single nanoparticles (polystyrene latex particles, TiO(2), Au, Mn, quantum dots, and carbon nanotubes) can be delivered to cells and animals via the air. The degree of agglomeration can be controlled by changing the suspension feeding rate to simulate realistic conditions for exposure studies.

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    Article: Release of carbon nanotubes from an epoxy-based nanocomposite during an abrasion process.
    Lukas Schlagenhauf, Bryan T T Chu, Jelena Buha, Frank Nüesch, Jing Wang
    [show abstract] [hide abstract]
    ABSTRACT: The abrasion behavior of an epoxy/carbon nanotube (CNT) nanocomposite was investigated. An experimental setup has been established to perform abrasion, particle measurement, and collection all in one. The abraded particles were characterized by particle size distribution and by electron microscopy. The abrasion process was carried out with a Taber Abraser, and the released particles were collected by a tube for further investigation. The particle size distributions were measured with a scanning mobility particle sizer (SMPS) and an aerodynamic particle sizer (APS) and revealed four size modes for all measured samples. The mode corresponding to the smallest particle sizes of 300-400 nm was measured with the SMPS and showed a trend of increasing size with increasing nanofiller content. The three measured modes with particle sizes from 0.6 to 2.5 μm, measured with the APS, were similar for all samples. The measured particle concentrations were between 8000 and 20,000 particles/cm(3) for measurements with the SMPS and between 1000 and 3000 particles/cm(3) for measurements with the APS. Imaging by transmission electron microscopy (TEM) revealed that free-standing individual CNTs and agglomerates were emitted during abrasion.
    Environmental Science & Technology 06/2012; 46(13):7366-72. · 4.80 Impact Factor
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Keywords

aerosol delivery
 
airborne nanoparticles
 
carbon nanotubes
 
different sizes
 
difficult challenges
 
dispersion
 
dispersion method
 
nanoparticle dispersion system
 
nanoparticle toxicology
 
nanoparticles
 
polystyrene latex particles
 
quantum dots
 
simulate realistic conditions
 
single nanoparticles
 
spatial uniformity
 
vivo
 
vivo studies