The mechanical characteristics and in vitro biocompatibility of poly(glycerol sebacate)-Bioglass (R) elastomeric composites

Department of Materials Engineering, Monash University, Clayton, Victoria 3800, Australia.
Biomaterials (Impact Factor: 8.56). 11/2010; 31(33):8516-29. DOI: 10.1016/j.biomaterials.2010.07.105
Source: PubMed


Biodegradable elastomeric materials have gained much recent attention in the field of soft tissue engineering. Poly(glycerol sebacate) (PGS) is one of a new family of elastomers which are promising candidates used for soft tissue engineering. However, PGS has a limited range of mechanical properties and has drawbacks, such as cytotoxicity caused by the acidic degradation products of very soft PGS and degradation kinetics that are too fast in vivo to provide sufficient mechanical support to the tissue. However, the development of PGS/based elastomeric composites containing alkaline bioactive fillers could be a method for addressing these drawbacks and thus may pave the way towards wide clinical applications. In this study, we synthesized a new PGS composite system consisting of a micron-sized Bioglass filler. In addition to much improved cytocompatibility, the PGS/Bioglass composites demonstrated three remarkable mechanical properties. First, contrary to previous reports, the addition of microsized Bioglass increases the elongation at break from 160 to 550%, while enhancing the Young's modulus of the composites by up to a factor of four. Second, the modulus of the PGS/Bioglass composites drops abruptly in a physiological environment (culture medium), and the level of drop can be tuned such that the addition of Bioglass does not harden the composite in vivo and thus the desired compliance required for soft tissue engineering are maintained. Third, after the abrupt drop in modulus, the composites exhibited mechanical stability over an extended period. This latter observation is an important feature of the new composites, because they can provide reliable mechanical support to damaged tissues during the lag phase of the healing process. These mechanical properties, together with improved biocompatibility, make this family of composites better candidates than plastic and related composite biomaterials for the applications of tissue engineering.

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    • "To overcome these limitations, making a composite with bioceramics of PGS could be a potential strategy. For example, the investigation of PGS- Bioglass Ò composites developed by Liang et al. (2010) showed that the addition of Bioglass Ò filler to PGS could be a control of degradation kinetics, which is independent of the mechanical properties of the composites. In addition, the composites have significantly improved biocompatibility compared with pure PGS. "
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    12/2014; 3(2-4):61-102. DOI:10.1007/s40204-014-0026-7
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    • "A standard cytotoxicity assessment method set by the International Organization of Standardization (ISO 10993) was used. Similar procedures have been published elsewhere [35] [36]. Briefly, extractant media are prepared by soaking the test or control materials in standard cell culture media (10% foetal calf serum, 1% L-glutamine and 0.5% penicillin/streptomycin) at a concentration of 0.2 g of material per ml of culture medium for 24 h at 37 • C and 5% CO 2 conditions in an incubator. "
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    • "Manufacture of binary or more complex biocompatible hybrid materials requires a compromise between specific mechanical performance, integrity and biological properties [1] [2] [3]. In particular , synthesis of enzymatic-cleavable material of an enhanced filler-matrix interaction is an important aspect of the biocomposites science [4] [5] [6] [7]. "
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