Article

Plant Transformation: Needs and Futurity of the Transgenes

Biotechnology 01/2008; DOI:http://www.doaj.org/doaj?func=openurl&genre=article&issn=1682296X&date=2008&volume=7&issue=3&spage=403
Source: DOAJ

ABSTRACT To produce transgenic plants which have various applications in agricultural and non-agricultural fields, a marker gene is necessary to recover a viable transgenic plant. To express or transcribe of transgenes, utilization of promoters is also unavoidable. Analysis of transgenes includes copy number, insertion site, integration stability, expression and it`s pattern and variability is immensely important in order to develop a successful transgenic event. This review presents the necessities for better recovery of transgenic plants, transcription or expression of transgenes, as well as methods to analyze transgenes.

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Keywords

copy number
 
insertion site
 
integration stability
 
it`s pattern
 
necessities
 
non-agricultural fields
 
successful transgenic event
 
transgenic plants
 
utilization
 
viable transgenic plant