Article

High-throughput analysis of hexosamine using a colorimetric method.

Department of Biological and Agricultural Engineering, University of California, Davis, 95616, USA.
Analytical Biochemistry (impact factor: 3). 01/2011; 408(1):160-2. DOI:10.1016/j.ab.2010.08.022 pp.160-2
Source: PubMed

ABSTRACT A 96-well plate method was developed for analysis of total hexosamine content in biological samples. Four hexosamine monomer derivatives-glucosamine hydrochloride, glucosamine sulfate, galactosamine hydrochloride, and mannosamine hydrochloride-were examined for the linearity of their spectra in the concentration range specified in the assay. The hexosamine concentration analysis range was linear from 0.1 to 1 mM. The quantification of hexosamines from chitin and chitosan upon acid hydrolysis was also tested. Accurate quantification of glucosamine content in chitin and chitosan with different molecular sizes and degrees of acetylation was demonstrated using the new method.

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Keywords

96-well plate method
 
Accurate quantification
 
acid hydrolysis
 
chitin
 
degrees
 
different molecular sizes
 
galactosamine hydrochloride
 
glucosamine content
 
hexosamine monomer derivatives-glucosamine hydrochloride
 
mannosamine hydrochloride-were
 
new method
 
quantification
 
spectra
 
total hexosamine content