Neutral and acidic oligosaccharides in Holstein-Friesian colostrum during the first 3 days of lactation measured by high performance liquid chromatography on a microfluidic chip and time-of-flight mass spectrometry

Department of Food Science and Technology, University of California, Davis 95616, USA.
Journal of Dairy Science (Impact Factor: 2.57). 09/2010; 93(9):3940-9. DOI: 10.3168/jds.2010-3156
Source: PubMed

ABSTRACT Oligosaccharides (OS) from bovine milk are a class of bioactive molecules that are receiving increasing commercial attention for their potential health benefits. In the present work we measured, comprehensively and systematically, free milk OS in the colostrum of 7 Holstein-Friesian cows during the first 3 d of lactation in 12-h intervals by HPLC-chip/time-of-flight mass spectrometry to determine the biological variation of free milk OS in early lactation. The high sensitivity and resolution of the analytical technique made it possible to monitor all OS species, thus providing a comprehensive and quantitative analysis of OS variations during colostrum production. This study confirmed that although sialyllactose is the major OS in bovine colostrum, several neutral OS species are present in significant abundance even at the third day of lactation. Furthermore, variation in terms of OS species and relative abundances of OS between cows suggest individual animal variation. These variations are likely due to genetic factors because environmental factors such as nutrition, lactation number, and accommodation were the same for all cows. This investigation revealed that colostrum milk from Holstein-Friesian cows is a rich source of neutral and acidic OS for the food and pharmaceutical industries.

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Available from: Daniela Barile, May 14, 2014
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    • "Until recently, the analysis of oligosaccharides from bovine milk was hindered by the lack of effective analytical tools. Recently, the lower abundant oligosaccharides from bovine milk were characterized and shown to contain complex structures similar to HMO (Barile et al., 2010; Hua et al., 2012; Wickramasinghe et al., 2011). There are 40 unique BMO in bovine colostrum, including 24 acidic structures containing sialic acid, and 16 neutral oligosaccharide structures containing glucose, galactose, and N-acetylglucosamine (Tao et al., 2009). "
    Encyclopedia of Agriculture and Food Systems, Edited by Van Alfen NK, 07/2014: chapter Determining Functional Properties and Sources of Recently Identified Bioactive Food Components: Oligosaccharides, Glycolipids, Glycoproteins, and Peptides: pages 441-461; Academic Press., ISBN: 978-0-08-093139-5
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    • "Conversely, neutral oligosaccharides are more difficult to detect in negative mode because their ionization efficiency is lower, in fact neutral oligosaccharides have a low tendency to form [M-H]-. Therefore, to improve neutral oligosaccharides detection, we formed a metal-carbohydrate adduct using Sodium, and then performed the analyses in positive ion-detection mode, forming the adduct [M+Na]+. The spectrum of the neutral oligosaccharides found in the mother liquor powder is shown in Figure 2. Bovine oligosaccharides previously reported in whey [22] and milk/colostrum [16], [17], [19] were observed (i.e. those between 500–1000 m/z in Figure 2). "
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    ABSTRACT: Oligosaccharides are the third largest component in human milk. This abundance is remarkable because oligosaccharides are not digestible by the newborn, and yet they have been conserved and amplified during evolution. In addition to encouraging the growth of a protective microbiota dominated by bifidobacteria, oligosaccharides have anti-infective activity, preventing pathogens from binding to intestinal cells. Although it would be advantageous adding these valuable molecules to infant milk formula, the technologies to reproduce the variety and complexity of human milk oligosaccharides by enzymatic/organic synthesis are not yet mature. Consequently, there is an enormous interest in alternative sources of these valuable oligosaccharides. Recent research has demonstrated that bovine milk and whey permeate also contain oligosaccharides. Thus, a thorough characterization of oligosaccharides in bovine dairy streams is an important step towards fully assessing their specific functionalities. In this study, bovine milk oligosaccharides (BMOs) were concentrated by membrane filtration from a readily available dairy stream called "mother liquor", and analyzed by high accuracy MALDI FT-ICR mass spectrometry. The combination of HPLC and accurate mass spectrometry allowed the identification of ideal processing conditions leading to the production of Kg amount of BMO enriched powders. Among the BMOs identified, 18 have high-molecular weight and corresponded in size to the most abundant oligosaccharides present in human milk. Notably 6 oligosaccharides contained fucose, a sugar monomer that is highly abundant in human milk, but is rarely observed in bovine milk. This work shows that dairy streams represent a potential source of complex milk oligosaccharides for commercial development of unique dairy ingredients in functional foods that reproduce the benefits of human milk.
    PLoS ONE 05/2014; 9(5):e96040. DOI:10.1371/journal.pone.0096040 · 3.23 Impact Factor
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    • "and analysed using a nano-chip liquid chromatography combined with high-performance mass spectrometry (Agilent 6520 Accurate-Mass-Quadrupole-Time-of-Flight , Agilent Technologies, Santa Clara, CA) according to the method previously reported (Barile et al., 2010; Ninonuevo et al., 2006). The micro fluidic "
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    ABSTRACT: Hazelnut skins are a good example of agricultural by-product with the potential to become a valuable source of functional ingredients. In this work, the fibre from hazelnut skins was extracted by using water and alkali solution and characterised by a suite of analytical tools (MALDI-FTICR, nano LC-Chip-Q-ToF and gas chromatography). Over thirty complex free oligosaccharides, composed mainly of galacturonic acid and N-acetylgalactosamine, were characterised for the first time in the present study. Their concentration ranged between 16 and 34mg per g of extract. The oligosaccharides isolated from this agricultural by-product are mainly hexose oligosaccharides (potentially galacto-oligosaccharides,) and xyloglucans. The identified composition could justify the bioactive activity of the extracts, namely prebiotic activity, previously demonstrated.
    Food Chemistry 10/2013; 140(4):717-25. DOI:10.1016/j.foodchem.2013.01.061 · 3.39 Impact Factor
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