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Neutral and acidic oligosaccharides in Holstein-Friesian colostrum during the first 3 days of lactation measured by high performance liquid chromatography on a microfluidic chip and time-of-flight mass spectrometry

Department of Food Science and Technology, University of California, Davis 95616, USA.
Journal of Dairy Science (Impact Factor: 2.55). 09/2010; 93(9):3940-9. DOI: 10.3168/jds.2010-3156
Source: PubMed

ABSTRACT Oligosaccharides (OS) from bovine milk are a class of bioactive molecules that are receiving increasing commercial attention for their potential health benefits. In the present work we measured, comprehensively and systematically, free milk OS in the colostrum of 7 Holstein-Friesian cows during the first 3 d of lactation in 12-h intervals by HPLC-chip/time-of-flight mass spectrometry to determine the biological variation of free milk OS in early lactation. The high sensitivity and resolution of the analytical technique made it possible to monitor all OS species, thus providing a comprehensive and quantitative analysis of OS variations during colostrum production. This study confirmed that although sialyllactose is the major OS in bovine colostrum, several neutral OS species are present in significant abundance even at the third day of lactation. Furthermore, variation in terms of OS species and relative abundances of OS between cows suggest individual animal variation. These variations are likely due to genetic factors because environmental factors such as nutrition, lactation number, and accommodation were the same for all cows. This investigation revealed that colostrum milk from Holstein-Friesian cows is a rich source of neutral and acidic OS for the food and pharmaceutical industries.

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Available from: Daniela Barile, May 14, 2014
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    • "Until recently, the analysis of oligosaccharides from bovine milk was hindered by the lack of effective analytical tools. Recently, the lower abundant oligosaccharides from bovine milk were characterized and shown to contain complex structures similar to HMO (Barile et al., 2010; Hua et al., 2012; Wickramasinghe et al., 2011). There are 40 unique BMO in bovine colostrum, including 24 acidic structures containing sialic acid, and 16 neutral oligosaccharide structures containing glucose, galactose, and N-acetylglucosamine (Tao et al., 2009). "
    Encyclopedia of Agriculture and Food Systems, Edited by Van Alfen NK, 07/2014: chapter Determining Functional Properties and Sources of Recently Identified Bioactive Food Components: Oligosaccharides, Glycolipids, Glycoproteins, and Peptides: pages 441-461; Academic Press., ISBN: 978-0-08-093139-5
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    • "and analysed using a nano-chip liquid chromatography combined with high-performance mass spectrometry (Agilent 6520 Accurate-Mass-Quadrupole-Time-of-Flight , Agilent Technologies, Santa Clara, CA) according to the method previously reported (Barile et al., 2010; Ninonuevo et al., 2006). The micro fluidic "
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