Oriented Insertion of phi29 N-Hexahistidine-tagged gp10 Connector Protein Assemblies into C(20)BAS Bolalipid Membrane Vesicles

Department of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, Indiana 47907.
Journal of the American Chemical Society (Impact Factor: 12.11). 08/2010; 132(48). DOI: 10.1021/ja104204z
Source: PubMed


Ni(2+):NTA-PEG600-grafted glass surfaces are capable of immobilizing N-his(6) gp10 connector protein assemblies from the phi29 DNA packaging motor and mediating their transplantation into bolalipid vesicles whose membrane thickness is compatible with the hydrophobic domain of the gp10 assemblies.

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