Article

Observing photophysical properties of quantum dots in air at the single molecule level: advantages in microarray applications.

Biological College, State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha, Hunan 410082, China.
Lab on a Chip (impact factor: 5.67). 11/2010; 10(21):2844-7. DOI:10.1039/c005258b pp.2844-7
Source: PubMed

ABSTRACT Quantum dots (QDs) are promising fluorescent tags for microarrays. Because most microarrays are analyzed under dry conditions, it is necessary to examine the photo properties of QDs in air. We demonstrate that the photophysical characteristics of individual quantum dots are different at the liquid/solid interface compared with QDs at the air/solid interface by observing them through a wide-field fluorescence microscope. QDs in air show higher photo-stability, higher fluorescence signal, slower spectral blue shift rate, less blinking and shorter bulk fluorescence lifetime than those in solution. These beneficial properties indicate QDs are good alternative fluorescent probes for microarrays.

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Keywords

air/solid interface
 
beneficial properties
 
higher fluorescence signal
 
higher photo-stability
 
individual quantum dots
 
liquid/solid interface
 
photophysical characteristics
 
Quantum dots
 
shorter bulk fluorescence lifetime
 
slower spectral blue shift rate
 
wide-field fluorescence microscope
 

Xingbo Shi