The inhibitory effects of Gelam honey and its extracts on nitric oxide and prostaglandin E(2) in inflammatory tissues.

Department of Anesthesiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
Fitoterapia (Impact Factor: 2.23). 12/2010; 81(8):1196-201. DOI: 10.1016/j.fitote.2010.07.024
Source: PubMed

ABSTRACT We investigated the effects of honey and its methanol and ethyl acetate extracts on inflammation in animal models. Rats' paws were induced with carrageenan in the non-immune inflammatory and nociceptive model, and lipopolysaccharide (LPS) in the immune inflammatory model. Honey and its extracts were able to inhibit edema and pain in inflammatory tissues as well as showing potent inhibitory activities against NO and PGE(2) in both models. The decrease in edema and pain correlates with the inhibition of NO and PGE(2). Phenolic compounds have been implicated in the inhibitory activities. Honey is potentially useful in the treatment of inflammatory conditions.

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    ABSTRACT: Tualang honey ( Fēng Mì) is known to have anti-inflammatory property, but its antinociceptive property has not been extensively investigated. In this study, we examined the preemptive effects on administering different doses of Tualang honey and prednisolone on the nociceptive response in male Sprague-Dawley rats. Thirty-five male Sprague-Dawley rats were randomized into five groups (n = 7) and each group received either distilled water, Tualang honey (0.2, 1.2 or 2.4 g/kg) or prednisolone (10 mg/kg) for 10 days. The response to noxious thermal stimulus was assessed using tail flick test on Day 10. The well-being of the rats was also assessed by monitoring their food intake and body weight. Data were analyzed using one-way Analysis of Variance (ANOVA) with post-hoc Scheffe's test and P value less than 0.05 was considered significant. In tail flick test, the tail flick latency time was significantly higher in the groups that received 1.2 g/kg and 2.4 g/kg of Tualang honey and 10 mg/kg of prednisolone, compared to the control group (P < 0.05). There was significant reduction in the total food pellet intake in the groups receiving prednisolone and Tualang honey (1.2 g/kg and 2.4 g/kg) compared to controls; however, the body weight gain was only significantly reduced in the prednisolone group. All the parameters were not significantly affected in the group receiving 0.2 g/kg of Tualang honey. In conclusion, preemptive administration of Tualang honey (1.2 g/kg and 2.4 g/kg) and prednisolone (10 mg/kg) had reduced the pain responses. The reduced weight gain in the prednisolone group is an unwanted effect due to its metabolic and central actions. Further studies are required to confirm the antinociceptive effects and elucidate the mechanism of antinociceptive action of Tualang honey in the rats.
    Journal of traditional and complementary medicine. 10/2014; 4(4):298-302.
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    ABSTRACT: Luteolin, a major component of flavones, is known to have various physiological properties. Although luteolin reportedly has an antinociceptive effect on acute and inflammatory pain, little is known about its effect on neuropathic pain. The aim of the present study was to determine whether luteolin could ameliorate hyperalgesia in the central nervous system using a neuropathic pain model. Chronic constriction injury to the sciatic nerve was induced in male Sprague–Dawley rats. Luteolin (0.1–1.5 mg) was administered intrathecally or intracerebroventricularly to examine the central effects on mechanical, thermal, and cold hyperalgesia using the electronic von Frey test, plantar test, and cold plate test, respectively. A rotarod test was also performed to assess motor function in normal rats. Spinally applied luteolin dose-dependently attenuated mechanical and cold hyperalgesia, but it had no effect on thermal hyperalgesia. At the highest dose, luteolin affected motor performance. The spinal action of luteolin on mechanical hyperalgesia was inhibited by intrathecal pretreatment with the γ-aminobutyric acidA (GABAA) receptor antagonist bicuculline and μ-opioid receptor antagonist naloxone, but not by intrathecal pretreatment with either the benzodiazepine receptor antagonist flumazenil or glycine receptor antagonist strychnine. Supraspinal application of luteolin had no antihyperalgesic effects in any test. These findings suggest that luteolin ameliorates mechanical and cold hyperalgesia at least in part by activating GABAA receptors in a flumazenil-insensitive manner and μ-opioid receptors in the spinal cord, but that the supraspinal regions are unlikely to contribute to the antihyperalgesic action of luteolin. Luteolin could be a candidate therapeutic agent for neuropathic pain.
    Pharmacology Biochemistry and Behavior 10/2014; · 2.82 Impact Factor
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    ABSTRACT: Introduction Exposure to stress during pregnancy has been shown to have adverse effects on pregnancy outcomes such as low birth weight, spontaneous abortion and premature delivery. Honey has been shown to improve testicular function in rats exposed to cigarette smoke. The aim of this study was to determine the effects of honey on corticosterone level, adrenal gland histomorphometry and pregnancy outcomes in pregnant rats subjected to restraint stress. Materials and methods Rat dams were divided into four groups (n = 10/group) i.e. control, honey, stress and honey plus stress (honey + stress) groups. Rats from honey and honey + stress groups received Tualang honey (1.2 g/kg body weight/day) orally by gavage from Day 0 of pregnancy until delivery. Rats from stress and honey + stress groups were subjected to stress by repeated restraining (three times/day) from Day 11 of pregnancy until delivery. Following delivery, pregnancy outcomes were assessed and dams were euthanized at postnatal Day 21 for assessments on serum corticosterone level, adrenal gland histology and rate of resorption. Results Rats from stress group had significantly higher corticosterone level, zona fasciculata thickness and duration of pregnancy as well as decreased litter size when compared with control and honey groups. These parameters were significantly improved in rats receiving honey (honey + stress group). Conclusion Supplementation of honey has a protective effect against increased corticosterone level and zona fasciculata thickness as well as impaired pregnancy outcomes in rats subjected to restraint stress.
    European Journal of Integrative Medicine 07/2014; · 0.65 Impact Factor


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May 27, 2014