Detection of waterborne parasites using field-portable and cost-effective lensfree microscopy

Electrical Engineering Department, UCLA, CA 90095, USA.
Lab on a Chip (Impact Factor: 6.12). 09/2010; 10(18):2419-23. DOI: 10.1039/c004829a
Source: PubMed


Protection of human health and well-being through water quality management is an important goal for both the developed and the developing parts of the world. In the meantime, insufficient disinfection techniques still fail to eliminate pathogenic contaminants in freshwater as well as recreational water resources. Therefore, there is a significant need for screening of water quality to prevent waterborne outbreaks and incidents of water-related diseases. Toward this end, here we investigate the use of a field-portable and cost-effective lensfree holographic microscope to image and detect pathogenic protozoan parasites such as Giardia Lamblia and Cryptosporidium Parvum at low concentration levels. This compact lensless microscope (O. Mudanyali et al., Lab Chip, 2010, 10, 1417-1428), weighing approximately 46 grams, achieves a numerical aperture of approximately 0.1-0.2 over an imaging field of view that is more than an order of magnitude larger than a typical 10X objective lens, and therefore may provide an important high-throughput analysis tool for combating waterborne diseases especially in resource limited settings.

15 Reads
  • Source
    • "[3] "
    [Show abstract] [Hide abstract]
    ABSTRACT: Lensfree on-chip microscopy is an emerging imaging technique that can be used to visualize and study biological specimens without the need for imaging lens systems. Important issues that can limit the performance of lensfree on-chip microscopy include interferometric aberrations, acquisition noise, and image reconstruction artifacts. In this study, we introduce a Bayesian-based method for performing aberration correction and numerical diffraction that accounts for all three of these issues to improve the effective numerical aperture (NA) and signal-to-noise ratio (SNR) of the reconstructed microscopic image. The proposed method was experimentally validated using the USAF resolution target as well as real waterborne Anabaena flos-aquae samples, demonstrating improvements in NA by ~25% over the standard method, and improvements in SNR of 2.3 dB and 3.8 dB in the reconstructed image when compared to the reconstructed images produced using the standard method and a maximum likelihood estimation method, respectively.
    Optics Letters 02/2015; 40(10). DOI:10.1364/OL.40.002233 · 3.29 Impact Factor
  • Source
    • "Over the last few years, computational lensfree on-chip imaging has emerged and promises to be a versatile, cost-effective, field-portable and yet powerful biomedical imaging tool both in labs and at remote sites89. Based on digital in-line holography, this on-chip imaging technique was shown to be an alternative tool for various clinical and telemedicine applications, addressing diagnosis of diseases such as malaria10, automated cell counting11, water quality monitoring12, blood analysis13 as well as imaging cytometry141516. In this work, we demonstrate that lensfree on-chip imaging can provide a solution to wide-field cell motility tracking that can overcome many of the above outlined shortcomings of lens-based optical microscopy methods. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Quantitative cell motility studies are necessary for understanding biophysical processes, developing models for cell locomotion and for drug discovery. Such studies are typically performed by controlling environmental conditions around a lens-based microscope, requiring costly instruments while still remaining limited in field-of-view. Here we present a compact cell monitoring platform utilizing a wide-field (24 mm(2)) lensless holographic microscope that enables automated single-cell tracking of large populations that is compatible with a standard laboratory incubator. We used this platform to track NIH 3T3 cells on polyacrylamide gels over 20 hrs. We report that, over an order of magnitude of stiffness values, collagen IV surfaces lead to enhanced motility compared to fibronectin, in agreement with biological uses of these structural proteins. The increased throughput associated with lensfree on-chip imaging enables higher statistical significance in observed cell behavior and may facilitate rapid screening of drugs and genes that affect cell motility.
    Scientific Reports 09/2014; 4:4717. DOI:10.1038/srep04717 · 5.58 Impact Factor
  • Source
    • "Recognizing this need, low-cost chip-scale microscopes have been developed over the last few years, with reliable performance and compact configurations suitable for field-diagnostic applications [5], [6], [7], [8], [9]. Our recent development, named the sub-pixel sweeping microscopy (SPSM) [6], has shown high-resolution (660 nm) and wide-field (5.7 mm×4.3 mm) imaging with a low-cost and compact geometry. "
    [Show abstract] [Hide abstract]
    ABSTRACT: We demonstrate a compact portable imaging system for the detection of waterborne parasites in resource-limited settings. The previously demonstrated sub-pixel sweeping microscopy (SPSM) technique is a lens-less imaging scheme that can achieve high-resolution (<1 µm) bright-field imaging over a large field-of-view (5.7 mm×4.3 mm). A chip-scale microscope system, based on the SPSM technique, can be used for automated and high-throughput imaging of protozoan parasite cysts for the effective diagnosis of waterborne enteric parasite infection. We successfully imaged and identified three major types of enteric parasite cysts, Giardia, Cryptosporidium, and Entamoeba, which can be found in fecal samples from infected patients. We believe that this compact imaging system can serve well as a diagnostic device in challenging environments, such as rural settings or emergency outbreaks.
    PLoS ONE 02/2014; 9(2):e89712. DOI:10.1371/journal.pone.0089712 · 3.23 Impact Factor
Show more


15 Reads
Available from