Article

A concept of dual optical detection using three light emitting diodes.

University of Warsaw, Department of Chemistry, Pasteura 1, 02-093 Warsaw, Poland.
Talanta (impact factor: 3.79). 06/2010; 82(1):422-5. DOI:10.1016/j.talanta.2010.04.026 pp.422-5
Source: PubMed

ABSTRACT In this paper a concept of very simple measurement system dedicated for simultaneous photometric and fluorometric detection is presented. Only three ordinary unmodified light emitting diodes (LEDs) can be applied in this analytical device: one of them is used in the conventional way as a source of nearly monochromatic light inducing fluorescence, whereas two others are applied as photometric and fluorometric detectors of light. In this study quinine is chosen as a model analyte. The reported device enables simultaneous detection of this analyte in the micromolar range of concentration. The practical utility of prototype dual detector for complex sample analysis is illustrated by its application for determination of quinine in tonic water samples.

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Keywords

analytical device
 
complex sample analysis
 
conventional way
 
fluorometric detection
 
fluorometric detectors
 
micromolar range
 
model analyte
 
monochromatic light inducing fluorescence
 
ordinary unmodified light emitting diodes
 
photometric
 
practical utility
 
prototype dual detector
 
reported device enables simultaneous detection
 
simple measurement system
 
simultaneous photometric
 
tonic water samples
 

Marta Pokrzywnicka