Efficacy of different commercial phytase enzymes and development of an available phosphorus release curve for Escherichia coli-derived phytases in nursery pigs

Department of Animal Sciences and Industry, College of Agriculture, Kansas State University, Manhattan, 66506, USA.
Journal of Animal Science (Impact Factor: 1.92). 11/2010; 88(11):3631-44. DOI: 10.2527/jas.2010-2936
Source: PubMed

ABSTRACT In 2 experiments, a total of 184 pigs (PIC, initial BW of 10.3 and 9.7 kg for Exp. 1 and 2, respectively) were used to develop an available P (aP) release curve for commercially available Escherichia coli-derived phytases. In both experiments, pigs were fed a corn-soybean meal basal diet (0.06% aP) and 2 diets with added inorganic P (iP) from monocalcium phosphate (Exp. 1: 0.075 and 0.15% aP; Exp. 2: 0.07 and 0.14% aP) to develop a standard curve. In Exp. 1, 100, 175, 250, or 500 phytase units (FTU)/kg of OptiPhos 2000 or 200, 350, 500, or 1,000 FTU/kg of Phyzyme XP were added to the basal diet. In Exp. 2, 250, 500, 750, or 1,000 FTU/kg of OptiPhos 2000; 500, 1,000, or 1,500 FTU/kg of Phyzyme XP; or 1,850 or 3,700 FTU/kg of Ronozyme P were added to the basal diet. One FTU was defined as the amount of enzyme required to release 1 µmol of iP per minute from sodium phytate at 37°C. For all phytase products, the manufacturer-guaranteed phytase activities were used in diet formulation. All diets were analyzed for phytase activity using both the Phytex and AOAC methods. Pigs were blocked by sex and BW and allotted to individual pens with 8 pens per treatment. Pigs were killed on d 21, and fibulas were collected and analyzed for bone ash. In both experiments, increasing iP improved (linear, P < 0.01) G:F and percentage bone ash. Pigs fed increasing OptiPhos had improved (Exp. 1: linear, P < 0.001; Exp. 2: quadratic, P < 0.001) percentage bone ash, as did pigs fed increasing Phyzyme XP (linear, P < 0.001). In Exp. 2, increasing Ronozyme P improved (quadratic, P < 0.01) percentage bone ash. Using analyzed values from the AOAC method and percentage bone ash as the response variable, an aP release curve was developed for up to 1,000 FTU/kg of E. coli-derived phytases (OptiPhos 2000 and Phyzyme XP) in P-deficient diets. The prediction equation was Y = -0.000000125X(2) + 0.000236X + 0.016, where Y = aP release (%) and X = analyzed phytase (FTU/kg) in the diet.

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    • "4 and 5). Thus, this provides a large variation (i.e., 25- 310% of enzyme recovery) in the reported data (i.e., Sulabo et al., 2011; Kerr et al., 2010; Jones et al., 2010; Yu et al., 2004). However, the universal methods were ring-trial validated: several laboratories analyzed activities of different enzyme products and various feed samples added with various products. "
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    ABSTRACT: In order to evaluate in vitro enzyme activity and in vivo efficacies of three new phytase products the diets of CON, Phy-500, Phy-1000 and Phy-5000 which were formulated to contain 0.0, 500, 1000 and 5000 FTU phytases per kg diet, respectively were analyzed for their phytase activity by a universal method and fed to the 14 day-old quail chicks for 3 weeks. The results indicated that the universal method was successfully transferred at our laboratory (the precision of measurements=1.6-2.6%). The measurements obtained from this study were more precise and accurate than any other data reported previously. However, the attention must be made on less recovery (39-40%) with thermostable enzyme. The results of efficacy trial indicated that weight gain and feed conversion ratio (FCR) were high with all enzyme added diets during feeding period compared to CON diet. The Phy-1000, however, provided significantly better FCR values than the rest of diets due to a marked reduction (P<0.05) in feed intake. Dry matter retention was significantly (P<0.05) high with Phy-500 diet. Weight (g) and relative weight (g/100 BW) of digestive tract was significantly (P<0.05) low with Phy-1000 and Phy-5000 diets. The length of
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    • "Exogenous phytases preparations are probably one of the most commonly used feed additives in the feed industry and many phytase products are commercially available. Moreover, each year novel generations of the enzyme come onto the market but there is a wide variation in the recommended dosages of enzyme necessary for similar P release from feeds (Jones et al., 2010). Thus, potential users can be confused about the efficacy as well as about a proper application of a particular enzyme. "
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    ABSTRACT: The effect of different phytases on the performance response, nutrient utilization and tibia characteristics of chickens was investigated. The five pelleted diets were the following: positive control (PC) with added monocalcium phosphate; negative control (NC) formulated with equivalency values of phytase for Ca and digestible P; and three further diets where different phytases were individually added to the NC diet at 500 FTU/kg. The phytases were derived either from Aspergillus (phytase I), or E. coli (phytases II and III). As compared to PC, the performance parameters, as well as AMEn, mineral retention, bone breaking force and tibia mineral content were suppressed by the reduction of dietary Ca and digestible P. All phytases enhanced the overall body weight gains and feed conversion ratio in comparison with NC, but none outperformed PC. Only phytase II improved AMEn as compared to NC and PC group. However only phytase I outperformed NC group in terms of mineral retention, and in P retention was higher than also phytase II and III. No significant differences were observed in fat digestibility and N retention. Bone strength among phytases did not differ, and all improved this parameter as compared to the NC diet. However, even though all phytases enhanced tibia minerals content, the improvement was less pronounced with phytase III. Moreover, the differences in all analysed tibia minerals between phytase III and II were significant suggesting that even among 6-phytases derived from and expressed in the same organism, different efficacy or mode of action can occur.
    Archiv fur Tierzucht 11/2013; 56. DOI:10.7482/0003-9438-56-104 · 0.33 Impact Factor
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    • "The three commonly used phytase feed enzymes are derived from Aspergillus niger, which is a 3-phytase and Peniophora lycii and Escherichia coli, which are 6-phytases [7]. A number of studies compared different sources of exogenous phytase in pigs and observed differences in physico-chemical characteristics [10,11] and efficacy [12,13]. Recently, a novel bacterial 6-phytase (Ronozyme HiPhos, DSM Nutritional Products, Parsippany, NJ) expressed in Aspergillus oryzae was developed, but there is no information on the effectiveness of this phytase when fed to pigs. "
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    ABSTRACT: In 2 experiments, 48 weanling (initial BW: 13.5 ± 2.4 kg, Exp. 1) and 24 growing pigs (initial BW: 36.2 ± 4.0 kg, Exp. 2) were used to determine effects of a novel bacterial 6-phytase expressed in Aspergillus oryzae on the apparent total tract digestibility (ATTD) of phosphorus and calcium in corn-soybean meal diets fed to weanling and growing pigs. In Exp. 1 and 2, pigs were randomly allotted to 6 dietary treatments using a randomized complete block design and a balanced 2 period changeover design, respectively. In both experiments, 6 diets were formulated. The positive control diet was a corn-soybean meal diet with added inorganic phosphorus (Exp. 1: 0.42 and 0.86% standardized total tract digestible phosphorus and total calcium, respectively; Exp. 2: 0.32 and 0.79% standardized total tract digestible phosphorus and total calcium, respectively). A negative control diet and 4 diets with the novel phytase (Ronozyme HiPhos, DSM Nutritional Products Inc., Parsippany, NJ) added to the negative control diet at levels of 500, 1,000, 2,000, and 4,000 phytase units (FYT)/kg were also formulated. In Exp. 1, the ATTD of phosphorus was greater (P < 0.01) for the positive control diet (60.5%) than for the negative control diet (40.5%), but increased (linear and quadratic, P < 0.01) as phytase was added to the negative control diet (40.5% vs. 61.6%, 65.1%, 68.7%, and 68.0%). The breakpoint for the ATTD of phosphorus (68.4%) was reached at a phytase inclusion level of 1,016 FYT/kg. In Exp. 2, the ATTD of phosphorus was greater (P < 0.01) for the positive control diet (59.4%) than for the negative control diet (39.8%) and increased (linear and quadratic, P < 0.01) as phytase was added to the negative control diet (39.8% vs. 58.1%, 65.4%, 69.1%, and 72.8%). The breakpoint for the ATTD of phosphorus (69.1%) was reached at a phytase inclusion level of 801 FYT/kg. In conclusion, the novel bacterial 6-phytase improved the ATTD of phosphorus and calcium in both weanling and growing pigs. The optimum level of inclusion for this phytase is 800 to 1,000 FYT/kg of complete feed to maximize ATTD of phosphorus and calcium in weanling and growing pigs.
    03/2013; 4(1):8. DOI:10.1186/2049-1891-4-8
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