Clinical validation of an autoantibody test for lung cancer

International Prevention Research Institute, Lyon, France.
Annals of Oncology (Impact Factor: 7.04). 02/2011; 22(2):383-9. DOI: 10.1093/annonc/mdq361
Source: PubMed


Autoantibodies may be present in a variety of underlying cancers several years before tumours can be detected and testing for their presence may allow earlier diagnosis. We report the clinical validation of an autoantibody panel in newly diagnosed patients with lung cancer (LC).
Three cohorts of patients with newly diagnosed LC were identified: group 1 (n = 145), group 2 (n = 241) and group 3 (n = 269). Patients were individually matched by gender, age and smoking history to a control individual with no history of malignant disease. Serum samples were obtained after diagnosis but before any anticancer treatment. Autoantibody levels were measured against a panel of six tumour-related antigens (p53, NY-ESO-1, CAGE, GBU4-5, Annexin 1 and SOX2). Assay sensitivity was tested in relation to demographic variables and cancer type/stage.
The autoantibody panel demonstrated a sensitivity/specificity of 36%/91%, 39%/89% and 37%/90% in groups 1, 2 and 3, respectively, with good reproducibility. There was no significant difference between different LC stages, indicating that the antigens included covered the different types of LC well.
This assay confirms the value of an autoantibody panel as a diagnostic tool and offers a potential system for monitoring patients at high risk of LC.

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Available from: Caroline J Chapman, Jan 10, 2014
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    • "and specificity of 94.9% (95% CI, 89.4–97.8%). The diagnostic accuracy of autoantibodies against NY-ESO-1 in NPC was greater than those previously detected in breast cancer, lung cancer and neuroblastoma (10,11,24,28,29). "
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    Oncology letters 09/2014; 8(3):1096-1102. DOI:10.3892/ol.2014.2286 · 1.55 Impact Factor
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    • "We had access to a well characterised cohort of sera from healthy volunteers and at risk individuals, thereby enabling crucial age- and gender- matching of HCC samples, and analysis of autoantibody patterns in important at-risk groups. We were also able to use a technically and clinically validated assay platform technology thereby ensuring autoantibody assays were conducted in a highly reproducible manner [32], [33]. We note that Zhang et al, and Chen et al. reported sensitivities of 67% to a panel of 10 antigens [27], [28], however, their failure to use appropriately age- and sex-matched controls leaves a significant clinically relevant question on their assay performance unanswered. "
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    PLoS ONE 08/2014; 9(8):e103867. DOI:10.1371/journal.pone.0103867 · 3.23 Impact Factor
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    • "Multiplex the tumor-associated antigens have been extensively explored by ELISA [32], [33], [34], [35], [36] or microarray [37]. This study is the first report by combining Luminex platform and HaloTag technology to detect humoral immune response in lung cancer patients. "
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    PLoS ONE 04/2014; 9(4):e95444. DOI:10.1371/journal.pone.0095444 · 3.23 Impact Factor
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