Protective Role of Moringa oleifera (Sajina) Seed on Arsenic-Induced Hepatocellular Degeneration in Female Albino Rats

Department of Bio-Medical Laboratory Science and Management, UGC Innovative Department, Vidyasagar University, Midnapore, 721 102 West Bengal, India.
Biological trace element research (Impact Factor: 1.75). 08/2011; 142(2):200-12. DOI: 10.1007/s12011-010-8761-7
Source: PubMed


In an attempt to develop new herbal therapy, an aqueous extract of the seed of Moringa oleifera was used to screen the effect on arsenic-induced hepatic toxicity in female rat of Wistar strain. Subchronic exposure to sodium arsenite (0.4 ppm/100 g body weight/day via drinking water for a period of 24 days) significantly increased activities of hepatic and lipid function markers such as alanine transaminase, aspartate transaminase, cholesterol, triglycerides, LDL along with a decrease in total protein and HDL. A notable distortion of hepatocellular histoarchitecture was prominent with a concomitant increase in DNA fragmentation following arsenic exposure. A marked elevation of lipid peroxidation in hepatic tissue was also evident from the hepatic accumulation of malondialdehyde and conjugated dienes along with suppressed activities in the antioxidant enzymes such as superoxide dismutase and catalase. However, co-administration of aqueous seed extract of M. oleifera (500 mg/100 g body weight/day for a period of 24 days) was found to significantly prevent the arsenic-induced alteration of hepatic function markers and lipid profile. Moreover, the degeneration of histoarchitecture of liver found in arsenic-treated rats was protected along with partial but definite prevention against DNA fragmentation induction. Similarly, generation of reactive oxygen species and free radicals were found to be significantly less along with restored activities of antioxidant enzymes in M. oleifera co-administered group with comparison to arsenic alone treatment group. The present investigation offers strong evidence for the hepato-protective and antioxidative efficiencies of M. oleifera seed extract against oxidative stress induced by arsenic.

1 Follower
57 Reads
  • [Show abstract] [Hide abstract]
    ABSTRACT: The present study elucidated the protective role of vitamin B(12) with folic acid against arsenic-induced hepatotoxicity in female rats. Ingestion of sodium-arsenite- contaminated water [0.4 ppm/100 g body weight (b.w.)/day] in combination with vitamin B(12) plus folic acid (0.07 and 4.0 μg, respectively/100 g b.w./day) for 24 days to Wistar rats offered a significant protection against alone arsenic-induced distorted liver function, damaged histoarchitecture, elevated oxidative stress, and DNA fragmentation of hepatic tissues. Arsenic only exposure decreased hepatic superoxide dismutase (SOD), catalase activities, and the level of nonprotein-soluble thiol (NPSH), with a concomitant increase in thiobarbituric acid reactive substances (TBARS) and conjugated dienes (CDs) in the liver. Vitamin supplementation restrained the increase of TBARS and CDs by restoring catalase, SOD, and NPSH levels. Restricted generation of free radicals may be correlated to the protection of DNA stability and hepatic morphology. This study explains the decisive role of vitamin B(12) with folic acid to ameliorate arsenic-mediated liver injuries.
    Drug and Chemical Toxicology 08/2011; 35(1):81-8. DOI:10.3109/01480545.2011.589439 · 1.23 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The prophylactic efficacy of Moringa oleifera, activated charcoal and wood charcoal to reduce lead toxicity in Wistar rats was evaluated. Seventy-five rats were divided into five groups consisting of 15 rats and marked as groups I to V. Group I was given distilled water while groups II to V were given lead acetate at a daily dose of 1000 mg/kg for seven days consecutively. Group II served as positive control while group I was a negative control. Groups III, IV and V were subsequently treated daily with extract leaves of Moringa oleifera, activated charcoal and wood charcoal respectively, at a dose of 1000 mg/kg for a period of one week (n = 5) and two weeks (n = 5). Group II was given distilled water daily for two weeks to study the possibility of natural recovery. The adverse effects of lead toxicity on biochemical and hematological parameters were demonstrated when the positive groups where compared to negative groups. Treatment with Moringa oleifera, activated charcoal but not wood charcoal, resulted in improvement in biochemical and ematological parameters. This study demonstrates ameliorating effects of Moringa oleifera on lead induced toxicities in the liver, kidneys, and blood, there is potential to exploit these properties for treatment and prevention. Keywords: Wistar rats, lead acetate, Moringa oleifera, activated charcoal, wood charcoal
    Medycyna weterynaryjna 02/2012; 68(2):96-101. · 0.22 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Anoma moringa Lour., Guilandina moringa Linn., Hyperanthera arborea J.F. Gmel., Hyperanthera decandra Willd., Hyperanthera moringa Vahl, Moringa erecta Salisb., Moringa moringa Millsp., Moringa octogona Stokes, Moringa parvifolia Noronha, Moringa polygona DC., Moringa pterygosperma C.F. Gaertn, Moringa nux-ben Perr., Moringa zeylanica Pers.
    Edible Medicinal And Non Medicinal Plants, 01/2012: pages 453-485; , ISBN: 978-94-007-2533-1
Show more