Dietary Blueberries Attenuate Atherosclerosis in Apolipoprotein E-Deficient Mice by Upregulating Antioxidant Enzyme Expression

USDA Arkansas Children's Nutrition Center, University of Arkansas for Medical Sciences, Little Rock, AR 72202, USA.
Journal of Nutrition (Impact Factor: 3.88). 09/2010; 140(9):1628-32. DOI: 10.3945/jn.110.123927
Source: PubMed


Protective effects of blueberries (BB) against atherosclerosis and potential underlying mechanisms in reducing oxidative stress were examined in apoE-deficient (apoE(-/-)) mice. ApoE(-/-) mice were fed an AIN-93G diet (CD) or CD formulated to contain 1% freeze-dried whole BB for 20 wk. The mean lesion area for apoE(-/-) mice fed BB was reduced by 39% (P < 0.001) in the aorta sinus and 58% (P < 0.001) in the descending aorta compared with CD-fed mice. These atheroprotective effects were independent of the serum lipid profile or total antioxidant capacity (as measured by oxygen radical absorbance capacity). The concentration of a biomarker of lipid peroxidation, F(2)-isoprostane, was lower in liver of BB-fed mice (P < 0.05). Genes analyzed by RT-PCR array showed that 4 major antioxidant enzymes in aorta [superoxide dismutase (SOD) 1, SOD2, glutathione reductase (GSR), and thioredoxin reductase 1] were upregulated in BB-fed mice. Enzyme activities of SOD and GSR were greater (P < 0.05) in liver and/or serum of BB-fed mice than those of CD-fed mice. In addition, serum paraoxonase 1 activity in serum of BB-fed mice was also greater than that of CD-fed mice (P < 0.05) at the end of the study. These results suggest a protective effectiveness of BB against atherosclerosis in this apoE(-/-) mouse model. The potential mechanisms may involve reduction in oxidative stress by both inhibition of lipid peroxidation and enhancement of antioxidant defense.

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    • "Fresh blueberries are known to have higher antioxidant capacity than other fruits (Wu et al., 2004). Phytochemicals from blueberries are reported to lower blood cholesterol (Prior et al., 2009) and prevent cancers and atherosclerosis (Adams et al., 2010; Wu et al., 2010). Research suggested that phytochemicals extracted from blueberries were more effective than whole fruits in preventing body weight gain (Prior et al., 2008). "
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    ABSTRACT: Blueberries contain antioxidant phytochemicals, such as anthocyanins, flavonols, and procyanidins, with many reported health benefits. In the present study, phytochemicals from blueberries were extracted using ultra-sound assisted hot water extraction and concentrated with Amberlite adsorption resins. Static adsorption tests showed that FPX66 resin had a higher adsorption capacity and desorption ratio than XAD7HP and XAD4 resins. XAD761 and XAD1180 showed the lowest desorption capacity and ratio. Kinetic adsorption and isotherm tests revealed that FPX66 had the highest adsorption efficiency and required shorter time to reach adsorption equilibrium. Dynamic adsorption on a FPX 66 resin column demonstrated that anthocyanins in the blueberry water extract started to break through after 16 bed volumes of extract was loaded. A complete desorption was achieved using 3 bed volumes of 95% ethanol. One hundred grams of fresh blueberries yielded 0.80 g concentrated blueberry extract. Sugars were not detected in the extract.
    Journal of Food Engineering 04/2014; 128:167-173. DOI:10.1016/j.jfoodeng.2013.12.029 · 2.77 Impact Factor
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    • "Blueberries are sold fresh or processed as individually quick frozen (IQF) fruit, juice or dried or infused berries, which in turn may be used in a variety of consumer products such as jellies, jams, pies, muffins, snack foods and cereals. Blueberries have been shown to reduce the risk of artherosclerosis in apolipoprotein-E deficient mice, which are highly prone to oxidative stress and antioxidant deficiencies in situations of high blood cholesterol [4]. Blueberry extract was found to be effective in inhibiting proliferation of HL60 human leukemia and HCT116 human colon carcinoma cells in vitro. "
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    • "However, experimental diet FBL seemed to counteract the adverse effect of L-NAME on the ALAT increase, suggesting a possible protecting effect on hepatocytes. Consumption of blueberries has been reported to reduce hepatocyte injury, lipid peroxidation [24], and oxidative stress [25, 26]. Furthermore, fermentation of blueberries has been shown to increase the total polyphenols and antioxidant capacities [27, 28]. "
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