Antifungal activity of extracts of some plants used in Brazilian traditional medicine against the pathogenic fungus Paracoccidioides brasiliensis. Pharm Biol
ABSTRACT Paracoccidioidomycosis (PCM) is a systemic granulomatous disease caused by Paracoccidioides brasiliensis Almeida (Onygenales) that requires 1-2 years of treatment. In the absence of drug therapy, the disease is usually fatal, highlighting the need for the identification of safer, novel, and more effective antifungal compounds. With this need in mind, several plants employed in Brazilian traditional medicine were assayed on P. brasiliensis and murine macrophages. Extracts were prepared from 10 plant species: Inga spp. Mill. (Leguminosae), Schinus terebinthifolius Raddi (Anacardiaceae), Punica granatum L. (Punicaceae), Alternanthera brasiliana Kuntze (Amaranthaceae), Piper regnellii CDC. (Piperaceae), P. abutiloides Kunth (Piperaceae), Herissantia crispa L. Briz. (Malvaceae), Rubus urticaefolius Poir (Rosaceae), Rumex acetosa L. (Polygonaceae), and Baccharis dracunculifolia DC. (Asteraceae). Hexane fractions from hydroalcoholic extracts of Piper regnellii and Baccharis dracunculifolia were the most active against the fungus, displaying minimum inhibitory concentration (MIC) values of 7.8 microg/mL and 7.8-30 mug/mL, respectively. Additionally, neither of the extracts exhibited any apparent cytotoxic effects on murine macrophages at 20 microg/mL. Analyses of these fractions using gas chromatography-mass spectrometry (GC-MS) showed that the major components of B. dracunculifolia were ethyl hydrocinnamate (14.35%) and spathulenol (16.02%), while the major components of the hexane fraction of Piper regnellii were 1-methoxy-4-(1-propenyl) benzene (21.94%) and apiol (21.29%). The activities of these fractions against P. brasiliensis without evidence of cytotoxicity to macrophages justify their investigation as a potential source of new chemical agents for the treatment of PCM.
- SourceAvailable from: Luciana A. R. S. Lima
[Show abstract] [Hide abstract]
- "The minimal inhibitory concentration (MIC) values were obtained for the broth microdilution testing performed in accordance with the guidelines from the CLSI M27-A2 document (NCCLS, 2002). The assays with P. brasiliensis were performed according to the described methods (Johann et al., 2010). Amphotericin B and trimethoprimsulfamethoxazole were included as positive antifungal controls, and the stock solutions were prepared in dimethylsulfoxide and water, respectively . "
ABSTRACT: In this study, 9-hydroxy-folianin and sucrose octaacetate were isolated from the seed ethanol extract of Annona cornifolia A. St. -Hil. (Annonaceae). We found that 9-hydroxy-folianin was able to inhibit several clinical strains of the pathogenic fungus Paracoccidioides brasiliensis. The minimal inhibitory concentrations of 9-hydroxy-folianin against twelve clinical strains of P. brasiliensis were found to be in the range between 3.4 and 27.7μg mL−1 and were much more potent than the commercial antifungal trimethropin-sulfamethoxazole. P. brasiliensis isolates (Pb-18, Pb-11, Pb-01, Pb-B339, Pb-8 and Pb-18 virulent) were also susceptible to sucrose octaacetate. The growth of Candida albicans, C. tropicalis, C. parapsilosis and Cryptococcus gattii were not affected by these compounds. Sucrose octaacetate and 9-hydroxy-folianin showed no effects with amphotericin B and trimethropin-sulfamethoxazole, but they displayed a synergetic effect with itraconazole.Food Research International 08/2011; 44(7):2283-2288. DOI:10.1016/j.foodres.2010.11.030 · 3.05 Impact Factor
[Show abstract] [Hide abstract]
- "The treatment of this mycosis is long (1-2 years) or even more. Johann et al. (2010) studying the activity of extracts of some plants used in Brazilian traditional medicine against the pathogenic fungus that causes this mycosis, Paracoccidioides brasiliensis, reported that the hexane fraction from stems of P. granatum exhibited better antifungal activity against the three clinical isolated than other parts of the plant or other fractions of the same plant. Nevertheless other extracts of plants revealed to be significantly more effective against that fungus than P. granatum. "
ABSTRACT: The pomegranate, Punica granatum L., which can be found throughout the Mediterranean region, in Southeast Asia, California and Arizona in USA was in ancient times referred as possessing powers of fertility, abundance and good luck (fruit). The biological properties of extracts (antimicrobial, antioxidant, anticancer, anti-inflammatory, among other properties) obtained from several parts of pomegranate is reported in the present work. Due to such properties, the extracts have been used in therapeutics, such as in the prevention of infection, inflammation, cancer, among other applications. However, other aspects are also referred in the present work such as the good practices of culture and fruit preservation, search of new compounds, selection of cultivars through biotechnological techniques for obtaining juice or fruits ready to eat. Such compilation of information was based on the search in the ISI Web of Knowledge (Thomson Reuters) from 2009 up to the beginning of October 2010.
- [Show abstract] [Hide abstract]
ABSTRACT: Fatty acids are abundant in vegetable oils. They are known to have antibacterial and antifungal properties. Antifungal susceptibility was evaluated by broth microdilution assay following CLSI (formerly the NCCLS) guidelines against 16 fungal strains of clinical interest. In this work, fatty acid methyl esters (FAME) was able to inhibit 12 clinical strains of the pathogenic fungus Paracoccidioides brasiliensis and were also active in the bioautographic assay against Cladosporium sphaerospermum. FAME was a more potent antifungal than trimethoprim-sulfamethoxazole against P. brasiliensis under the experimental conditions tested.Revista da Sociedade Brasileira de Medicina Tropical 01/2011; 44(6):777-80. DOI:10.1590/S0037-86822011000600024 · 0.94 Impact Factor