Application of Broad-Spectrum Resequencing Microarray for Genotyping Rhabdoviruses

Institut Pasteur, Lyssavirus Dynamics and Host Adaptation Unit, Paris, France.
Journal of Virology (Impact Factor: 4.65). 09/2010; 84(18):9557-74. DOI: 10.1128/JVI.00771-10
Source: PubMed

ABSTRACT The rapid and accurate identification of pathogens is critical in the control of infectious disease. To this end, we analyzed the capacity for viral detection and identification of a newly described high-density resequencing microarray (RMA), termed PathogenID, which was designed for multiple pathogen detection using database similarity searching. We focused on one of the largest and most diverse viral families described to date, the family Rhabdoviridae. We demonstrate that this approach has the potential to identify both known and related viruses for which precise sequence information is unavailable. In particular, we demonstrate that a strategy based on consensus sequence determination for analysis of RMA output data enabled successful detection of viruses exhibiting up to 26% nucleotide divergence with the closest sequence tiled on the array. Using clinical specimens obtained from rabid patients and animals, this method also shows a high species level concordance with standard reference assays, indicating that it is amenable for the development of diagnostic assays. Finally, 12 animal rhabdoviruses which were currently unclassified, unassigned, or assigned as tentative species within the family Rhabdoviridae were successfully detected. These new data allowed an unprecedented phylogenetic analysis of 106 rhabdoviruses and further suggest that the principles and methodology developed here may be used for the broad-spectrum surveillance and the broader-scale investigation of biodiversity in the viral world.

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Available from: Jean-Claude Manuguerra, Jan 25, 2015
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    • "(Dacheux et al., 2010 "
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    • "A resequencing microarray was used in parallel to the bacteriological methods. This molecular tool was developed for the detection of a massive panel of viral and bacterial agents as well as more than 600 genes involved in pathogenicity or antibiotic resistance [6] [7] [8] [9]. Bacterial signatures obtained from the isolate confirmed the bacterial identification. "
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