Sophorolipid production by Candida bombicola on oils with a special fatty acid composition and their consequences on cell viability

Laboratory of Industrial Microbiology and Biocatalysis, Department of Biochemical and Microbial Technology, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000, Ghent, Belgium.
Biotechnology Letters (Impact Factor: 1.59). 10/2010; 32(10):1509-14. DOI: 10.1007/s10529-010-0323-8
Source: PubMed


Sophorolipids production by the yeast Candia bombicola is most favourable when glucose is used as a carbon source in combination with a hydrophobic carbon source such as a common vegetable oil. Most vegetable oils are comprised of C16-C18 fatty acids, an ideal range for sophorolipid production. The use of oils with either shorter or longer fatty acids, such has coconut oil or meadowfoam oil, respectively, was evaluated. Such oils did not contribute to enhanced sophorolipid production when compared to cultures run on glucose as the sole carbon source. Moreover, a toxic effect of medium-chain fatty acids towards stationary C. bombicola cells was demonstrated.

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    • "This is best represented by cultivation of the yeast in absence of any hydrophobic carbon source such that sophorolipid synthesis occurs merely de novo from glucose. In this case, about 20% of the sophorolipids contain a C16:0 lipid tail, while 46 and 34% contain a C18:1 and C18:0 fatty acid tail, respectively (Van Bogaert et al. 2010). The fatty acid composition is on the one hand governed by the specificity of the cytochrome P450 monooxygeanse CYP52M1, converting the common fatty acid to a terminal or subterminal hydroxylated one. "
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