Analysis of the contribution of acid phosphatase to P efficiency in Brassica napus under low phosphorus conditions
National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, China.Science China. Life sciences (Impact Factor: 1.69). 06/2010; 53(6):709-17. DOI: 10.1007/s11427-010-4008-2
To understand whether genotypic variation in acid phosphatase (APase) activity in rapeseed (Brassica napus L.) induced by phosphorus (P) deficiency has impact on P efficiency, soil APase activity in the rhizosphere for rapeseed P-efficient genotype 102 and P-inefficient genotype 105 was measured against organic and inorganic P sources in the pot experiment, and the activities of root-secreted APase and leaf intracellular APase were investigated in different P-starvation periods in the nutrient solution. Higher activity of root-secreted APase in B. napus was induced under low P conditions. However, P nutrition and P uptake efficiency of the plants supplied with organic P were not directly related to the activity of root-secreted APase due to several confounding factors affecting APase availability. The higher activity of leaf APase improved P remobilization in plants and played important roles in enhancing P use efficiency, shown by the significant correlation between leaf APase activity and P use efficiency in a rapeseed recombinant inbred population of 135 lines.
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ABSTRACT: Acid phosphatase (APase) is very important in phosphorus (P) scavenging and remobilization in plants. The aim of this study was the fine mapping of quantitative trait loci (QTL) for APase activity (APA) in maize (Zea mays L.) leaf. The QTL for APA were studied in the F2:3 population derived from the cross 082 × Ye107 under low P stress in two sites. A significant difference in APA was found between 082 (P-efficient genotype) and Ye107 (P-deficient genotype). Each environment was analyzed to identify the QTL. Six QTL for APA were found, comprising two QTL at Beibei (BB) and four QTL at Hechuan (HC), China. A QTL denoted as AP9 showed a stable expression under different environments on chromosome 9, and explained 10.21 and 16.81 % of phenotypic variation at BB and HC, respectively. For the fine mapping of this QTL, seven individuals selected via marker-assisted selection in the BC3F1 population were used to produce the BC3F2 lines by selfing and to allow recombination within the region containing the target QTL. High-resolution genetic and physical maps were further constructed for the fine mapping of AP9 using 12 simple sequence repeat markers and the BC3F2 population consisting of 1,441 individuals. As a result, the location of AP9 was narrowed down to a 546-kb fragment on chromosome 9.Molecular Breeding 10/2013; 32(3). DOI:10.1007/s11032-013-9895-z · 2.25 Impact Factor
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ABSTRACT: A series of 12 new dibemethin (N-benzyl-N-methyl-1-phenylmethanamine) derivatives bearing an N-aminomethyl group attached to the one phenyl ring and an H, Cl, OCH3 or N(CH3)2 group on the other have been synthesized. These compounds all showed strong chloroquine chemosensitizing activity, comparable to verapamil, when present at 1 μM in an in vitro culture of the chloroquine-resistant W2 strain of the human malaria parasite, Plasmodium falciparum. Their N-formylated derivatives also exhibited resistance-reversing activity, but only at substantially higher IC10 concentrations. A number of the dibemethin derivatives were shown to inhibit chloroquine transport via the parasite's 'chloroquine resistance transporter' (PfCRT) in a Xenopus laevis oocyte expression system. The reduced resistance-reversing activity of the formylated compounds relative to their free amine counterparts can probably be ascribed to two factors: decreased accumulation of the formylated dibemethins within the parasite's internal digestive vacuole (believed to be the site of action of chloroquine), and a reduced ability to inhibit PfCRT. The resistance-reversing activity of the compounds described here demonstrates that the amino group need not be attached to the two aromatic rings via a three or four carbon chain as has been suggested by previous QSAR studies. These compounds may be useful as potential side chains for attaching to a 4,7-dichloroquinoline group in order to generate new resistance-reversing chloroquine analogues with inherent antimalarial activity.European Journal of Medicinal Chemistry 02/2011; 46(5):1729-42. DOI:10.1016/j.ejmech.2011.02.026 · 3.45 Impact Factor
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ABSTRACT: Iron and phosphorus availability is low in many soils; hence, microorganisms and plants have evolved mechanisms to acquire these nutrients by altering the chemical conditions that affect their solubility. In plants, this includes exudation of organic acid anions and acidification of the rhizosphere by release of protons in response to iron and phosphorus deficiency. Grasses (family Poaceae) and microorganisms further respond to Fe deficiency by production and release of specific chelators (phytosiderophores and siderophores, respectively) that complex Fe to enhance its diffusion to the cell surface. In the rhizosphere, the mutual demand for Fe and P results in competition between plants and microorganisms with the latter being more competitive due to their ability to decompose plant-derived chelators and their proximity to the root surface; however microbial competitiveness is strongly affected by carbon availability. On the other hand, plants are able to avoid direct competition with microorganisms due to the spatial and temporal variability in the amount and composition of exudates they release into the rhizosphere. In this review, we present a model of the interactions that occur between microorganisms and roots along the root axis, and discuss advantages and limitations of methods that can be used to study these interactions at nanometre to centimetre scales. Our analysis suggests mechanisms such as increasing turnover of microbial biomass or enhanced nutrient uptake capacity of mature root zones that may enhance plant competitiveness could be used to develop plant genotypes with enhanced efficiency in nutrient acquisition. Our model of interactions between plants and microorganisms in the rhizosphere will be useful for understanding the biogeochemistry of P and Fe and for enhancing the effectiveness of fertilization.Soil Biology and Biochemistry 05/2011; 43(5):883-894. DOI:10.1016/j.soilbio.2011.01.005 · 3.93 Impact Factor
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