Article

Impact of chemokine receptor CX3CR1 in human renal allograft rejection.

Department of Internal Medicine II-Nephrology, University Medical Center Regensburg, Franz-Josef-Strauss-Allee 11, 93053 Regensburg, Germany.
Transplant Immunology (impact factor: 1.46). 08/2010; 23(4):204-8. DOI:10.1016/j.trim.2010.06.006 pp.204-8
Source: PubMed

ABSTRACT Chemokine receptors play pivotal roles for leukocyte recruitment in acute and chronic inflammatory processes. This study was performed to analyze the expression, distribution and cellular localization of CX3CR1 in human renal transplant biopsies and to assess its role as potential diagnostic and prognostic marker. CX3CR1 was prospectively analyzed in 174 renal graft biopsies from patients with normal morphology (n=76), antibody-mediated acute rejection (n=6), acute tubulointerstitial rejection (n=27), acute vascular rejection (n=31), and with acute tubulus necrosis (n=34). Double immunofluorescence was additionally performed for CX3CR1 and CD4, CD8, CD20, CD68, and CD209/DC-SIGN. The number of CX3CR1 positive interstitial cells was significantly higher in the biopsies with acute tubulointerstitial and acute vascular rejection as compared to normal renal allograft biopsies. CX3CR1 positive cells were mainly CD68 positive monocytes/macrophages and CD209/DC-SIGN positive dendritic cells. The percentage of the CX3CR1 positive staining area was a predictor for steroid responsiveness and for worse clinical outcome 3 and 12 months after transplantation. CX3CR1 positive macrophages and/or dendritic cells are significantly elevated in acute renal allograft rejection. As CX3CR1 was associated with outcome parameters, it has to be further evaluated as a prognostic marker in human renal transplantation.

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    PLoS ONE 01/2011; 6(10):e26663. · 4.09 Impact Factor
Data provided are for informational purposes only. Although carefully collected, accuracy cannot be guaranteed. The impact factor represents a rough estimation of the journal's impact factor and does not reflect the actual current impact factor. Publisher conditions are provided by RoMEO. Differing provisions from the publisher's actual policy or licence agreement may be applicable.

Keywords

174 renal graft biopsies
 
acute renal allograft rejection
 
acute tubulointerstitial
 
acute tubulointerstitial rejection
 
acute tubulus necrosis
 
acute vascular rejection
 
antibody-mediated acute rejection
 
CD209/DC-SIGN positive dendritic cells
 
cellular localization
 
chronic inflammatory processes
 
CX3CR1 positive cells
 
CX3CR1 positive interstitial cells
 
CX3CR1 positive macrophages
 
CX3CR1 positive staining area
 
human renal transplant biopsies
 
human renal transplantation
 
normal renal allograft biopsies
 
outcome parameters
 
prognostic marker
 
worse clinical outcome 3