Article

Cytoplasm and organelle transfer between mesenchymal multipotent stromal cells and renal tubular cells in co-culture.

A.N.Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow 119992, Russian Federation.
Experimental Cell Research (impact factor: 3.58). 09/2010; 316(15):2447-55. DOI:10.1016/j.yexcr.2010.06.009 pp.2447-55
Source: PubMed

ABSTRACT Cell-to-cell interactions of human mesenchymal multipotent stromal cells (MMSC) and rat renal tubular cells (RTC) were explored under conditions of co-cultivation. We observed formation of different types of intercellular contacts, including so called tunneling nanotubes. These contacts were shown to be able to provide transfer of cell's contents, including organelles. We documented intercellular exchange with fluorescent probes specific to cytosol, plasmalemma and mitochondria. Initial transport of cellular components was revealed after 3 h of co-culturing, and occurred in two directions--both direct and retrograde as referred to RTC. However, transport of probes toward MMSC was more efficient. One significant result of such transport was appearance of renal-specific Tamm-Horsfall protein in MMSC, indicating induction of their differentiation into kidney tubular cells. We conclude that transfer of cellular compartments between renal and stem cells could provide differentiation of MMSC when transplanted into kidney and result in therapeutic benefits in renal failure.

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Keywords

able
 
cell's contents
 
Cell-to-cell interactions
 
cellular compartments
 
co-cultivation
 
different types
 
efficient
 
fluorescent probes specific
 
human mesenchymal multipotent stromal cells
 
intercellular contacts
 
kidney tubular cells
 
mitochondria
 
MMSC
 
rat renal tubular cells
 
renal failure
 
renal-specific Tamm-Horsfall protein
 
significant result
 
therapeutic benefits
 
tunneling nanotubes