Potential of the TCE-degrading endophyte Pseudomonas putida W619-TCE to improve plant growth and reduce TCE phytotoxicity and evapotranspiration in poplar cuttings.
ABSTRACT The TCE-degrading poplar endophyte Pseudomonas putida W619-TCE was inoculated in poplar cuttings, exposed to 0, 200 and 400 mg l(-1) TCE, that were grown in two different experimental setups. During a short-term experiment, plants were grown hydroponically in half strength Hoagland nutrient solution and exposed to TCE for 3 days. Inoculation with P. putida W619-TCE promoted plant growth, reduced TCE phytotoxicity and reduced the amount of TCE present in the leaves. During a mid-term experiment, plants were grown in potting soil and exposed to TCE for 3 weeks. Here, inoculation with P. putida W619-TCE had a less pronounced positive effect on plant growth and TCE phytotoxicity, but resulted in strongly reduced amounts of TCE in leaves and roots of plants exposed to 400 mg l(-1) TCE, accompanied by a lowered evapotranspiration of TCE. Dichloroacetic acid (DCAA) and trichloroacetic acid (TCAA), which are known intermediates of TCE degradation, were not detected.
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ABSTRACT: The aim of this study was to determine whether the inoculation of plant growth-promoting bacteria to plants, vegetated in soil irrigated with textile effluent, influences plant biomass production, and soil remediation. Three different plant species (Acacia ampliceps, Eucalyptus camaldulensis, and Leucaena leucocephala) with and without bacterial inoculation were grown in soil irrigated with secondary treated textile wastewater for one year. An endophytic bacterium, Burkholderia sp. strain PsJN, possessing plant growth-promoting 1-aminocyclopropane-1-carboxylate deaminase activity was inoculated to plants. There was more plant biomass production (up to 12%) and contaminants removal (up to 29%) from soil with bacterial inoculation as compared to soil having non-inoculated plants. Enhanced plant growth and soil remediation activity are associated with the survival and colonization of the inoculated bacterium in the rhizosphere and endosphere of plants. The highest plant biomass production and contaminants removal from soil were observed in the treatment, in which A. ampliceps was inoculated with Burkholderia sp. strain PsJN. These results suggest that plant-bacteria partnerships can be applied to improve plant growth and soil remediation during the application of industrial effluent for plant biomass production in the arid regions.CLEAN - Soil Air Water 09/2014; 42(9). DOI:10.1002/clen.201300006 · 1.84 Impact Factor
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ABSTRACT: Many endophytes have been found to be resistant to heavy metals and/or capable of degrading organic contaminants, and endophyte-assisted phytoremediation has been documented as a promising technology for in situ remediation of contaminated soils. During the phytoremediation of heavy metals, the heavy-metal-resistant endophytes can enhance plant growth, decrease metal phytotoxicity, and affect metal translocation and accumulation in plants. For the phytoremediation of organic contaminants, endophytes can produce various enzymes to degrade organic contaminants and reduce both the phytotoxicity and evapotranspiration of volatile contaminants. This paper reviews the diversity of contaminant-resistant/degrading endophytes and their role in phytoremediation and discusses some issues that have been raised surrounding this area of research.Fungal diversity 05/2012; 54(1). DOI:10.1007/s13225-012-0165-x · 6.94 Impact Factor
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ABSTRACT: Phytoextraction has been reported as an economically and ecologically sound alternative for the remediation of metal-contaminated soils. Willow is a metal phytoextractor of interest because it allows to combine a gradual contaminant removal with production of biomass that can be valorized in different ways. In this work two willow clones growing on a metal-contaminated site were selected: 'Belgisch Rood' (BR) with a moderate metal extraction capacity and 'Tora' (TO) with a twice as high metal accumulation. All cultivable bacteria associated with both willow clones were isolated and identified using 16SrDNA ARDRA analysis followed by 16SrDNA sequencing. Further all isolated bacteria were investigated for characteristics that might promote plant growth (production of siderophores, organic acids and indol acetic acid) and for their metal resistance. The genotypic and phenotypic characterization of the isolated bacteria showed that the TO endophytic bacterial population is more diverse and contains a higher percentage of metal-resistant plant growth promoting bacteria than the endophytic population associated with BR. We hypothesize that the difference in the metal accumulation capacity between BR and TO clones might be at least partly related to differences in characteristics of their associated bacterial population.Microbial Biotechnology 02/2013; 6(3). DOI:10.1111/1751-7915.12038 · 3.21 Impact Factor