Article

Genistein aglycone improves skin repair in an incisional model of wound healing: a comparison with raloxifene and oestradiol in ovariectomized rats.

Department of Biochemical, Physiological and Nutritional Sciences, University of Messina, Messina, Italy.
British Journal of Pharmacology (impact factor: 4.41). 07/2010; 160(5):1185-94. DOI:10.1111/j.1476-5381.2010.00758.x pp.1185-94
Source: PubMed

ABSTRACT Oestrogen loss at menopause is frequently related to poor wound healing. Genistein has been tested in anti-ageing cosmetic preparations with interesting results on skin health. Here, we investigated the effects of the genistein aglycones, given systemically, in an incisional model of wound healing, compared to systemic oestradiol and raloxifene.
Six months after ovariectomy (OVX), rats were randomly assigned to groups of 12 animals each and treated daily with genistein aglycone (1 and 10 mg kg(-1) s.c.), raloxifene hydrochloride (0.05 and 0.5 mg kg(-1) s.c.) or 17-alpha-ethinyl oestradiol (0.003 and 0.03 mg kg(-1) s.c.) for 12 weeks. Untreated OVX and sham OVX rats were used as controls. Then, 14 or 7 days before the end of the experiment, an incisional wound healing procedure was performed and skin specimens were collected to evaluate molecular, histological and functional measurements.
Seven and fourteen days after wounding, samples from OVX rats showed a decrease in transforming growth factor-beta1, tissue transglutaminase 2 and vascular endothelial growth factor compared to samples from sham OVX rats. Oestradiol, raloxifene and genistein all significantly modified this decrease, but the lowest genistein dose exerted a greater effect than the other treatments. Moreover, the lowest dose of genistein was the most effective in improving skin healing and wound tensile strength.
Genistein aglycone might be an alternative therapy for the management of skin wound healing.

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    Article: In vivo performance of chitosan/soy-based membranes as wound dressing devices for acute skin wounds.
    Tírcia C Santos, Bernhard Hoering, Kathrin Reise, Alexandra Marques, Simone S Silva, Joaquim Miguel Oliveira, João Mano, António G Castro, Rui L Reis, Martijn van Griensven
    [show abstract] [hide abstract]
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    Tissue Engineering Part A 10/2012; · 4.64 Impact Factor
Data provided are for informational purposes only. Although carefully collected, accuracy cannot be guaranteed. The impact factor represents a rough estimation of the journal's impact factor and does not reflect the actual current impact factor. Publisher conditions are provided by RoMEO. Differing provisions from the publisher's actual policy or licence agreement may be applicable.

Keywords

17-alpha-ethinyl oestradiol
 
7 days
 
anti-ageing cosmetic preparations
 
genistein aglycones
 
greater effect
 
growth factor-beta1
 
incisional wound healing procedure
 
lowest dose
 
lowest genistein dose
 
Oestrogen loss
 
poor wound healing
 
sham OVX rats
 
skin healing
 
skin wound healing
 
systemic oestradiol
 
tissue transglutaminase 2
 
Untreated OVX
 
vascular endothelial growth factor
 
wound healing
 
wound tensile strength