Direct adhesion force measurements between E. coli and human uroepithelial cells in cranberry juice cocktail. Mol Nutr Food Res
Department of Chemical Engineering, Worcester Polytechnic Institute, Worcester, MA 01609, USA. Molecular Nutrition & Food Research
(Impact Factor: 4.6).
12/2010; 54(12):1744-52. DOI: 10.1002/mnfr.200900535
Atomic force microscopy (AFM) was used to directly measure the nanoscale adhesion forces between P-fimbriated Escherichia coli (E. coli) and human uroepithelial cells exposed to cranberry juice, in order to reveal the molecular mechanisms by which cranberry juice cocktail (CJC) affects bacterial adhesion.
Bacterial cell probes were created by attaching P-fimbriated E. coli HB101pDC1 or non-fimbriated E. coli HB101 to AFM tips, and the cellular probes were used to directly measure the adhesion forces between E. coli and uroepithelial cells in solutions containing: 0, 2.5, 5, 10, and 27 wt% CJC. Macroscale attachment of E. coli to uroepithelial cells was measured and correlated to nanoscale adhesion force measurements. The adhesion forces between E. coli HB101pDC1 and uroepithelial cells were dose-dependent, and decreased from 9.32±2.37 nN in the absence of CJC to 0.75±0.19 nN in 27 wt% CJC. Adhesion forces between E. coli HB101 and uroepithelial cells were low in buffer (0.74±0.18 nN), and did not change significantly in CJC (0.78±0.18 nN in 27 wt% CJC; P=0.794).
Our study shows that CJC significantly decreases nanoscale adhesion forces between P-fimbriated E. coli and uroepithelial cells.
Figures in this publication
Available from: Jessica A. Younes
- "For instance, after 120 s of bond maturation, Gardnerella vaginalis BME-1 adhered to the membrane region not located above the nucleus with an adhesion force of 11.9 nN versus 5.7 nN above the nucleus (significantly different at p < 0.05). For comparison, Liu et al. (2010) measured an adhesion force of 9.3 nN for Escherichia coli to uro-epithelial cells without making a distinction between membrane regions, in line with the values found here. "
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ABSTRACT: Vaginal epithelium is colonized by different bacterial strains and species. The bacterial composition of vaginal biofilms controls the balance between health and disease. Little is known about the relative contribution of the epithelial and bacterial cell surfaces to bacterial adhesion and whether and how adhesion is regulated over cell membrane regions. Here we show that bacterial adhesion forces with cell membrane regions not located above the nucleus are stronger than with regions above the nucleus both for vaginal pathogens and different commensal and probiotic lactobacillus strains involved in health. Importantly, adhesion force ratios over membrane regions away from and above the nucleus coincided with the ratios between numbers of adhering bacteria over both regions. Bacterial adhesion forces were dramatically decreased by depleting the epithelial cell membrane of cholesterol or sub-membrane cortical actin. Thus, epithelial cells can regulate membrane regions to which bacterial adhesion is discouraged, possibly to protect the nucleus. This article is protected by copyright. All rights reserved.
Cellular Microbiology 10/2015; DOI:10.1111/cmi.12537 · 4.92 Impact Factor
Available from: etd.uthsc.edu
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ABSTRACT: The Societe de Transport de l'Outaouais (STO) has acquired five
software packages to assist in its operations, and has made an effort to
fully integrate them. The Societe's initial acquisition was a software
package to allow it to plan transit service. The Societe installed an
automatic vehicle location system. A major component of this system is a
module which allows transit users, either at home or at the office, to
place a telephone call to know the actual arrival time for the next two
buses on each of the routes listed at any given bus stop. The Societe
has developed a software package to perform the daily and quarterly
assignments of its bus drivers more effectively. The Societe has
acquired a software package to assist its information agents to plan the
optimum bus routes, according to rider preferences. The STO's most
recent acquisition is an executive information system. This system,
based on the principle of management by exception, allows managers to
conduct daily and monthly monitoring of the performance indicators which
the STO has defined
Vehicle Navigation and Information Systems Conference, 1993., Proceedings of the IEEE-IEE; 11/1993
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