Direct adhesion force measurements between E. coli and human uroepithelial cells in cranberry juice cocktail
ABSTRACT Atomic force microscopy (AFM) was used to directly measure the nanoscale adhesion forces between P-fimbriated Escherichia coli (E. coli) and human uroepithelial cells exposed to cranberry juice, in order to reveal the molecular mechanisms by which cranberry juice cocktail (CJC) affects bacterial adhesion.
Bacterial cell probes were created by attaching P-fimbriated E. coli HB101pDC1 or non-fimbriated E. coli HB101 to AFM tips, and the cellular probes were used to directly measure the adhesion forces between E. coli and uroepithelial cells in solutions containing: 0, 2.5, 5, 10, and 27 wt% CJC. Macroscale attachment of E. coli to uroepithelial cells was measured and correlated to nanoscale adhesion force measurements. The adhesion forces between E. coli HB101pDC1 and uroepithelial cells were dose-dependent, and decreased from 9.32±2.37 nN in the absence of CJC to 0.75±0.19 nN in 27 wt% CJC. Adhesion forces between E. coli HB101 and uroepithelial cells were low in buffer (0.74±0.18 nN), and did not change significantly in CJC (0.78±0.18 nN in 27 wt% CJC; P=0.794).
Our study shows that CJC significantly decreases nanoscale adhesion forces between P-fimbriated E. coli and uroepithelial cells.
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Conference Paper: The use of computer for urban transit operations[Show abstract] [Hide abstract]
ABSTRACT: The Societe de Transport de l'Outaouais (STO) has acquired five software packages to assist in its operations, and has made an effort to fully integrate them. The Societe's initial acquisition was a software package to allow it to plan transit service. The Societe installed an automatic vehicle location system. A major component of this system is a module which allows transit users, either at home or at the office, to place a telephone call to know the actual arrival time for the next two buses on each of the routes listed at any given bus stop. The Societe has developed a software package to perform the daily and quarterly assignments of its bus drivers more effectively. The Societe has acquired a software package to assist its information agents to plan the optimum bus routes, according to rider preferences. The STO's most recent acquisition is an executive information system. This system, based on the principle of management by exception, allows managers to conduct daily and monthly monitoring of the performance indicators which the STO has definedVehicle Navigation and Information Systems Conference, 1993., Proceedings of the IEEE-IEE; 11/1993
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ABSTRACT: The glycosides of flavonoid, anthocyanins and A type proanthocyanidins in cranberry concentrate were characterized and quantified using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Cranberry concentrate (1 g/body weight) was orally gavaged to Fischer-344 rats (n = 6), and blood and urine samples were collected over 24 h periods. Quercetin, 3'-O-methylquercetin (isorhamnetin), myricetin, kaempferol, and proanthocyanidin dimer A2, together with thirteen conjugated metabolites of quercetin and methylquercetin and intact peonidin 3-O-galactoside and cyanidin 3-O-galactoside were identified in the rat urine after cranberry treatment. Very low levels of isorhamnetin (0.48 ± 0.09 ng/mL) and proanthocyanidin dimer A2 (0.541 ± 0.10 ng/mL) were found in plasma samples after 1 h of cranberry administration. Although no quercetin was detected in plasma, MRM analysis of the methanolic extract of urinary bladder showed that chronic administration of cranberry concentrate to rats resulted in accumulation of quercetin and isorhamnetin in the bladder. These results demonstrate that cranberry components undergo rapid metabolism and elimination into the urine of rats and are present in the urinary bladder tissue potentially allowing them to inhibit urinary bladder carcinogenesis.Journal of Agricultural and Food Chemistry 06/2011; 59(12):6682-8. DOI:10.1021/jf200673h · 3.11 Impact Factor