Distribution and characterization of Listeria monocytogenes clinical isolates in Portugal, 1994-2007

Centro de Biotecnologia e Química Fina, Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Dr António Bernardino de Almeida, 4200-072 Porto, Portugal.
European Journal of Clinical Microbiology (Impact Factor: 2.54). 10/2010; 29(10):1219-27. DOI: 10.1007/s10096-010-0988-x
Source: PubMed

ABSTRACT In recent years, the number of cases of listeriosis has increased worldwide. Ninety-five isolates of Listeria monocytogenes recovered from Portuguese human cases of listeriosis have been characterized by biotyping (cadmium and arsenic sensitivity), polymerase chain reaction (PCR) grouping, and by pulsed-field gel electrophoresis (PFGE) applying the enzymes AscI and ApaI. Isolates were classified into one of three PCR groups; IVb (71.6%), IIb (17.9%), and IIa (10.5%). Four biotypes were differentiated: sensitive to arsenic/cadmium (48.4%), arsenic-sensitive and cadmium-resistant (25.3%), resistant to arsenic and sensitive to cadmium (18.9%), and resistant to both heavy metals (7.4%). Combined analyses of AscI and ApaI patterns yielded a total of 58 PFGE types with five sets (G, Jb, KKa, Me, and U) of Portuguese strains, each of which were indistinguishable by PFGE typing. In the present study, it was demonstrated that there are recurrent pulsotypes and that some were the same pulsotypes linked to outbreaks in France. In addition, there are some pulsotypes spread throughout the country, while others only appear in a restricted region. This study allowed the assembly of a first large pulsotype database of Portuguese clinical strains.

Download full-text


Available from: Alexandre Leclercq, Jul 07, 2015
1 Follower
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Listeria was first described in 1926 by Murray, Webb, and Swann, who discovered it while investigating an epidemic infection among laboratory rabbits and guinea pigs. The role of Listeria monocytogenes as a foodborne pathogen was definitively recognized during the 1980s. This recognition was the consequence of a number of epidemic human outbreaks due to the consumption of contaminated foods, in Canada, in the USA and in Europe. Listeriosis is especially severe in immunocompromised individuals such as pregnant women. The disease has a low incidence of infection, although this is undeniably increasing, with a high fatality rate amongst those infected. In pregnant women listeriosis may cause abortion, fetal death, or neonatal morbidity in the form of septicemia and meningitis. Improved education concerning the disease, its transmission, and prevention measures for immunocompromised individuals and pregnant women has been identified as a pressing need.
    ISRN obstetrics and gynecology 09/2013; 2013:851712. DOI:10.1155/2013/851712
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Abuse, neglect and other forms of discrimination towards the elderly have always been present in history. There has been a model of "old person" in every culture, according to which older people are treated. The more idealized the model is, the less tolerant and more demanding society is towards the elderly. Presented results lead to the conclusion that significant number of older people feel mistreated because of their age in many aspects of daily life. Various forms of abuse may be difficult to thoroughly analyze, for they are so persistent in our collective consciousness. Unfortunately, material civilization progress is not sufficient to eliminate abuse. Thus, monitoring and relevant assistance to people exposed to abuse and violence, particularly those with compromised defense abilities, is crucial.
    Przegla̧d epidemiologiczny 01/2011; 65(3):503-7.
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Until now, few studies have focused on the relationship between place of living, the level of education and HIV infection in Poland. The anonymous questionnaires containing questions about place of living and level of education one year before diagnosis of HIV infection were distributed among HIV-infected patients during their meetings or were sent to patients of the HIV/AIDS reference treatment centres in Poland. Among patients who responded to questionnaire women contracting HIV infection through injecting psychoactive drugs and by heterosexual contacts predominate. Prior to diagnosis, most injection drug users who responded to questionnaire (66.4%) lived in villages and small towns (up to 100,000 inhabitants), usually with parents, and the majority (69.8%) were poorly educated (still continued or already completed primary or basic vocational school). Better educated were those who contracted the infection trough heterosexual contacts. In this group, no differences were observed in relation to the place of living. HIV infected men who have sex with men were better educated, most of them lived in Warsaw and other large cities. Despite all the limitations, the findings suggest that majority of HIV infections in Poland are acquired by young people living in small towns and villages who are poorly educated. So the most urgent need exist to enhance HIV prevention amongst young people in Poland.
    Przegla̧d epidemiologiczny 01/2011; 65(3):509-13.