Disease Genes and Gene Regulation by microRNAs

The John & Jennifer Ruddy Canadian Cardiovascular Genetics Centre, Ottawa, ON, Canada, K1Y 4W7.
Journal of Cardiovascular Translational Research (Impact Factor: 3.02). 06/2010; 3(3):169-72. DOI: 10.1007/s12265-010-9186-6
Source: PubMed
4 Reads
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Aging can be defined as a progressive decline in physiological efficiency regulated by an extremely complex multifactorial process. The genetic makeup of an individual appears to dictate this rate of aging in a species specific manner. For decades now, scientists have tried to look for tiny signatures or signs which might help us predict this rate of aging. MicroRNAs (miRNAs) are a unique class of short, non-coding RNAs that mediate the post-transcriptional regulation of gene expression ranging from developmental processes to disease induction or amelioration. Recently, they have also been implicated to have a role in aging in C.elegans. Based on the fact that there is a considerable similarity between aging in C.elegans and humans, these recent findings might suggest a possible role of miRNAs as bio-markers of aging. This mini-review brushes through the possibilities towards this direction.
    Bioinformation 02/2011; 5(9):396-7. DOI:10.6026/97320630005396 · 0.50 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Aims To investigate the effect of the microRNA-196a2 gene polymorphism (rs11614913) on risk of cardiovascular disease in type 2 diabetes patients. Methods We examined 920 patients with diabetes and 834 healthy controls. All subjects were genotyped for the miRNA-196a2 SNP by polymerase chain reaction (PCR) and restriction analysis. Results The genotype distribution among controls and patients was in Hardy-Weinberg equilibrium (p = 0.227 and 0.308, respectively). The frequency of the T allele was lower in patients than in controls (p = 0.044). The odds ratio 0.66 (95 % CI 0.54-0.79) suggests an association of the T allele with decreased risk of T2DM. For the main purpose of the study, T2DM patients were stratified into patients with CVD and those without it. The T allele and TT genotype were significantly more frequent in patients with CVD compared to those without CVD (p = 0.013, p < 0.001, respectively). The odds ratio for the T allele in the CVD + subgroup vs. CVD- was 1.76 (1.35-2.30), p < 0.0001, mostly due to the overrepresentation of TT homozygotes. The highest risk of development of CVD was observed in the additive model for TT homozygotes (OR 3.33, 95 % CI 2.05-5.42, p < 0.0001). Conclusion Our findings suggest that miRNA-196a2 T/C polymorphism (rs11614913) is associated with an increased risk of CVD in type 2 diabetes patients. This provides further insights on pathogenesis of cardiovascular disease in type 2 diabetes patients.
    Journal of diabetes and its complications 09/2014; 28(5). DOI:10.1016/j.jdiacomp.2014.05.006 · 3.01 Impact Factor


4 Reads
Available from