Fura-2FF-based calcium indicator for protein labeling

Laboratory of Protein Engineering, Institute of Chemical Sciences and Engineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH-1015, Lausanne, Switzerland.
Organic & Biomolecular Chemistry (Impact Factor: 3.56). 08/2010; 8(15):3398-401. DOI: 10.1039/c000158a
Source: PubMed


We describe the synthesis and fluorescence properties of a Fura-2FF-based fluorescent Ca(2+) indicator that can be covalently linked to SNAP-tag fusion proteins and retains its Ca(2+) sensing ability after coupling to protein.

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