Article

Determining UV inactivation of Toxoplasma gondii oocysts by using cell culture and a mouse bioassay.

National Exposure Research Laboratory, U.S. Environmental Protection Agency, Cincinnati, Ohio 45268, USA.
Applied and Environmental Microbiology (Impact Factor: 3.95). 08/2010; 76(15):5140-7. DOI: 10.1128/AEM.00153-10
Source: PubMed

ABSTRACT The effect of UV exposure on Toxoplasma gondii oocysts has not been completely defined for use in water disinfection. This study evaluated UV-irradiated oocysts by three assays: a SCID mouse bioassay, an in vitro T. gondii oocyst plaque (TOP) assay, and a quantitative reverse transcriptase real-time PCR (RT-qPCR) assay. The results from the animal bioassay show that 1- and 3-log(10) inactivation is achieved with 4 mJ/cm(2) UV and 10 mJ/cm(2) low-pressure UV, respectively. TOP assay results, but not RT-qPCR results, correlate well with bioassay results. In conclusion, a 3-log(10) inactivation of T. gondii oocysts is achieved by 10-mJ/cm(2) low-pressure UV, and the in vitro TOP assay is a promising alternative to the mouse bioassay.

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Available from: Swinburne A J Augustine, Jul 08, 2015
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    • "Parasite foci were detected after 10 days of incubation by crystal violet staining and counted as a correlate of oocyst (i.e. sporozoite) infectivity (Villegas et al. 2010; Ware et al. 2010). Formalin (10 %) completely prevented, and acidified Clorox R (10 %) considerably reduced parasite reproduction in the TOP assay (Villegas et al. 2010). "
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