Article

Salidroside induces cell-cycle arrest and apoptosis in human breast cancer cells

Department of Pathology and Pathophysiology, Zhejiang University School of Medicine, Hangzhou, China.
Biochemical and Biophysical Research Communications (Impact Factor: 2.28). 07/2010; 398(1):62-7. DOI: 10.1016/j.bbrc.2010.06.033
Source: PubMed

ABSTRACT Recently, salidroside (p-hydroxyphenethyl-beta-d-glucoside) has been identified as one of the most potent compounds isolated from plants of the Rhodiola genus used widely in traditional Chinese medicine, but pharmacokinetic data on the compound are unavailable. We were the first to report the cytotoxic effects of salidroside on cancer cell lines derived from different tissues, and we found that human breast cancer MDA-MB-231 cells (estrogen receptor negative) were sensitive to the inhibitory action of low-concentration salidroside. To further investigate the cytotoxic effects of salidroside on breast cancer cells and reveal possible ER-related differences in response to salidroside, we used MDA-MB-231 cells and MCF-7 cells (estrogen receptor-positive) as models to study possible molecular mechanisms; we evaluated the effects of salidroside on cell growth characteristics, such as proliferation, cell cycle duration, and apoptosis, and on the expression of apoptosis-related molecules. Our results demonstrated for the first time that salidroside induces cell-cycle arrest and apoptosis in human breast cancer cells and may be a promising candidate for breast cancer treatment.

Download full-text

Full-text

Available from: Xiaolan Hu, Nov 25, 2014
0 Followers
 · 
106 Views
  • Source
    • "It has exhibited a wide range of pharmacological properties, including anti-aging, anti-oxidation, anti-inflammatory, anti-fatigue, anti-depressant activities, antiviral effects, hepatoprotective, neuroprotective, and cardiovascular protective characteristics (Zhang et al., 2007, 2010; Wang et al., 2009). Recent studies have also shown that salidroside may prevent the growth of leukemia, stomach adenocarcinoma and parotid carcinoma (Hu et al., 2010), and may also significantly decrease neovascular reactions (De Bock et al., 2004). Despite its attractive pharmacological activities, the therapeutic potential of salidroside has been significantly restricted by its short biological half-life and poor oral bioavailability (Fan et al., 2007). "
    [Show abstract] [Hide abstract]
    ABSTRACT: A novel pH-responsive nano-carrier MSNs-PAA, possessing mesoporous silica nanoparticles (MSNs) cores and poly(acrylic acid) (PAA) shell-layers, was developed for controlled release of salidroside. The vinyl double bonds modified MSNs were synthesized by using cetyltrimethylammonium bromide (CTAB) as templates, tetraethyl orthosilicate (TEOS) as silicon source, and 3-(trimethoxylsilyl) propyl methacrylate (MPS) as surface modification functionalities. The pH-responsive layers of PAA were grafted onto the vinyl double bonds of the MSNs via precipitation polymerization, producing the MSNs-PAA with a hollow cubic core and mesoporous shell with penetrating pore channels. The characteristic results also showed that PAA was successfully grafted onto the surface of the MSNs. The MSNs-PAA was investigated as carriers for loading and regulating the release of salidroside in different pH solutions for the first time. The results demonstrated that the PAA layers on the surface of MSNs-PAA exhibited opened and closed states at different pH values, and thus could regulate the uptake and release of salidroside. The application of such pH-responsive nano-carrier might offer a potential platform for controlled delivery and increasing the bioavailability of drugs.
    International Journal of Pharmaceutics 02/2013; 446(1-2). DOI:10.1016/j.ijpharm.2013.01.071 · 3.65 Impact Factor
  • Source
    • "It is well known that cell growth and proliferation of mammalian cells are dependent on cell cycle progression (Schwartz and Shah, 2005) and the inhibition of the cell cycle has become an appreciated target for management and treatment of tumor cells with cytotoxic agents (Shih and Stutman, 1996; Schwartz and Shah, 2005). Many anti cancer agents and DNA-damaging agents arrest the cell cycle at the G 0 /G 1 , S, or G 2 /M phase and then induce apoptotic cell death (Purohit et al., 2000; Harada et al., 2002; Cheng et al., 2004; Sun and Liu, 2006; Hu et al., 2010). This current study showed that PWH arrested human breast cancer MCF-7 cells at S-G 2 /M phase. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Species of Phyllanthus have traditionally been used for hundreds of years for treating many ailments including diabetes, anemia, bronchitis and hepatitis. The present study aims to investigate the cytotoxic and apoptotic effects of methanol (PWM), hexane (PWH) and ethyl acetate (PWE) extracts from the leaves of the endemic plant Phyllanthus watsonii Airy Shaw (Phyllanthaceae) on MCF-7 human breast cancer cells. We observed that the PWM, PWH and PWE extracts were cytotoxic and selectively inhibited the growth and proliferation of MCF-7 cells compared to untreated control in a dose dependent manner with an IC(50) of 12.7±4.65, 7.9±0.60 and 7.7±0.29μg/ml, respectively. However, the extracts were not toxic at these concentrations to normal human lung fibroblast MRC-5 cells. Cell death induced by PWM, PWH and PWE extracts were mainly due to apoptosis which was characterized by apoptotic morphological changes and a nuclear DNA fragmentation. Caspase-3 activation following P. watsonii extracts treatment was also evident for apoptotic cell death which was preceded by an S phase cell cycle perturbation. The results suggested that the cytotoxic activity of P. watsonii extracts was related to an early event of cell cycle perturbation and a later event of apoptosis. Hence, P. watsonii displays potential to be further exploited in the discovery and development of new anticancer agents.
    Experimental and toxicologic pathology: official journal of the Gesellschaft fur Toxikologische Pathologie 01/2012; 65(3). DOI:10.1016/j.etp.2011.11.005 · 2.01 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Salidroside, a phenylpropanoid glycoside isolated from a traditional Chinese medicinal plant Rhodiola rosea L. displays a broad spectrum of pharmacological properties. It has been found to play a hepatoprotective role in liver diseases through inhibiting apoptosis of hepatocytes and proliferation of hepatic stellate cells, decreasing serum aminotransferase, reversing hepatic fibrosis, and improving liver function. In this study as an ongoing study on the discovery and development of new hepatoprotective agents, about 12 novel glycosides were synthesized, and 2,2-diphenyl-1-picrylhydrazyl radical scavenge activity of each glycoside was evaluated. 2-(3,4,5-trihydroxyphenyl)ethyl β-d-glucopyranoside (4g) and 2-(3,4,5-trihydroxyphenyl)ethyl β-d-galactopyranoside (4h) exhibited significant activity prior to salidroside with an IC50 value of 38.05 and 35.85 μM, respectively. The hepatoprotective effect of compounds 4g and 4h on CCl4-induced cytotoxicity in LO2 cells was assessed for further research. Graphical abstract 2-(3,4,5-Trihydroxyphenyl)ethyl β-d-glucopyranoside (4g) and 2-(3,4,5-trihydroxyphenyl)ethyl β-d-galactopyranoside (4h) exhibited significant hepatoprotective activity prior to salidroside.
    Medicinal Chemistry Research 05/2012; 22(5). DOI:10.1007/s00044-012-0247-z · 1.61 Impact Factor
Show more